Literature summary for 1.1.1.224 extracted from

Aly, R.; Cholakh, H.; Joel, D.M.; Leibman, D.; Steinitz, B.; Zelcer, A.; Naglis, A.; Yarden, O.; Gal-On, A.
Gene silencing of mannose 6-phosphate reductase in the parasitic weed Orobanche aegyptiaca through the production of homologous dsRNA sequences in the host plant (2009), Plant Biotechnol. J., 7, 487-498.

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Cloned(Commentary)

Cloned (Comment)	Organism
transgenic tomato plants bearing a gene construct containing a specific 277-bp fragment from M6PR-mRNA, in an inverted-repeat configuration. Eight independent lines containing the M6PR-silencing inverted-repeat construct pBin-IR-M6PR construct are regenerated	Phelipanche aegyptiaca

Inhibitors

Inhibitors	Comment	Organism	Structure
siRNA	transformed tomato plants containing siRNA from the M6PR of Orobanche aegyptiaca show normal growth and development, accumulation of M6PR siRNA in transgenic tomatoes correlates with decreased levels of M6PR mRNA. Amount of endogenous M6PR mRNA in the tubercles and underground shoots of Orobanche aegyptiaca grown on transgenic host plants is reduced by 60%-80%. Concomitant with M6PR mRNA suppression, there is a significant decrease in mannitol level and a significant increase in the percentage of dead Orobanche aegyptiaca tubercles on the transgenic host plants. Detection of mir390, which is involved with cytoplasmic dsRNA processing, indicates to the existence of gene-silencing mechanisms in Orobanche spp. Gene silencing mechanisms are probably involved with the production of decreased levels of M6PR mRNA in the parasites grown on the transformed tomato lines	Phelipanche aegyptiaca

Organism

Organism	UniProt	Comment	Textmining
Phelipanche aegyptiaca	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
root tubercle	-	Phelipanche aegyptiaca	-
shoot	underground	Phelipanche aegyptiaca	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-mannose 6-phosphate + NADPH + H+	-	Phelipanche aegyptiaca	D-mannitol 1-phosphate + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
M6PR	-	Phelipanche aegyptiaca
mannose 6-phosphate reductase	-	Phelipanche aegyptiaca

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Release 2023.1 (January 2023)