Any feedback?
Literature summary for 1.1.1.220 extracted from
- The biosynthesis of tetrahydrobiopterin in rat brain. Purification and characterization of 6-pyruvoyl tetrahydropterin (2-oxo)reductase (1989), J. Biol. Chem., 264, 8066-8073.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
6-pyruvoyltetrahydropterin | - |
Rattus norvegicus |  |
| 0.1 | - |
4-nitrobenzaldehyde | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
SDS-PAGE, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-pyruvoyltetrahydropterin + NADPH | Rattus norvegicus | involved in de novo biosynthesis of tetrahydrobiopterin | 6-lactoyl-5,6,7,8-tetrahydropterin + NADP+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from brain | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | highest concentration in striatum and midbrain | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22 | - |
crude extract | Rattus norvegicus |
| 49300 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrobenzaldehyde + NADPH | - |
Rattus norvegicus | 4-nitrobenzylalcohol + NADP+ | - |
? | |
| 6-pyruvoyltetrahydropterin + NADPH | - |
Rattus norvegicus | 6-lactoyl-5,6,7,8-tetrahydropterin + NADP+ | - |
ir | |
| 6-pyruvoyltetrahydropterin + NADPH | involved in de novo biosynthesis of tetrahydrobiopterin | Rattus norvegicus | 6-lactoyl-5,6,7,8-tetrahydropterin + NADP+ | - |
ir | |
| menadione + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| Phenanthrenequinone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | SDS-PAGE, gel filtration | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
