Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.22 extracted from

  • Loutet, S.A.; Bartholdson, S.J.; Govan, J.R.; Campopiano, D.J.; Valvano, M.A.
    Contributions of two UDP-glucose dehydrogenases to viability and polymyxin B resistance of Burkholderia cenocepacia (2009), Microbiology, 155, 2029-2039.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Ugd(BCAL2946) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAL2946) expressed in Escherichia coli HMS 174(DE3) Burkholderia cenocepacia J2315
gene Ugd(BCAM0855) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAM0855) expressed in Escherichia coli HMS 174(DE3) Burkholderia cenocepacia J2315
gene Ugd(BCAM2034) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAM2034) expressed in Escherichia coli BL21 (DE3) Burkholderia cenocepacia J2315

Protein Variants

Protein Variants Comment Organism
additional information inactivation of gene Ugd(BCAL2946) results in increased sensitivity to polymyxin B and this sensitivity can be overcome when either genes Ugd(BCAL2946) or Ugd(BCAM0855) but not gene ugd(BCAM2034) is expressed from plasmids. Growth of a conditional Ugd(BCAL2946) mutant, created in the DELTAUgd(BCAM0855) background, is significantly impaired under non-permissive conditions. Growth can be rescued by either Ugd(BCAL2946) or Ugd(BCAM0855) expressed in trans, but not by Ugd(BCAM2034) Burkholderia cenocepacia J2315

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
UDP-glucose
-
Burkholderia cenocepacia J2315
0.17
-
NAD+
-
Burkholderia cenocepacia J2315
0.21
-
NAD+
-
Burkholderia cenocepacia J2315

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50900
-
Ugd-FLAG-BCAM2034 Burkholderia cenocepacia J2315
51700
-
Ugd-FLAG-BCAL2946 Burkholderia cenocepacia J2315
52100
-
Ugd-FLAG-BCAM0855 Burkholderia cenocepacia J2315

Organism

Organism UniProt Comment Textmining
Burkholderia cenocepacia J2315 B4EB36
-
-
Burkholderia cenocepacia J2315 B4EMQ8
-
-
Burkholderia cenocepacia J2315 B4EN77
-
-

Purification (Commentary)

Purification (Comment) Organism
by nickel affinity and gel filtration Burkholderia cenocepacia J2315

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information expression of Ugd(BCAL2946) is 5.4- and 135fold greater than that of Ugd(BCAM0855) and Ugd(BCAM2034), respectively. Combined activity of Ugd(BCAL2946) and Ugd(BCAM0855) is essential for the survival of Burkholderia cenocepacia but only the most highly expressed ugd gene, Ugd(BCAL2946), is required for polymyxin B resistance. UDP-galactose, UDP-acetylglucosamine and GDP-mannose are not substrates Burkholderia cenocepacia J2315 ?
-
?
additional information expression of Ugd(BCAL2946) is 5.4- and 135fold greater than that of Ugd(BCAM0855) and Ugd(BCAM2034), respectively. Combined activity of Ugd(BCAL2946) and Ugd(BCAM0855) is essential for the survival of Burkholderia cenocepacia. UDP-galactose, UDP-acetylglucosamine and GDP-mannose are not substrates Burkholderia cenocepacia J2315 ?
-
?
additional information purified Ugd(BCAM2034) shows no in vitro Ugd activity. Expression of Ugd(BCAL2946) is 5.4- and 135fold greater than that of Ugd(BCAM0855) and Ugd(BCAM2034), respectively. UDP-galactose, UDP-acetylglucosamine and GDP-mannose are not substrates Burkholderia cenocepacia J2315 ?
-
?
UDP-glucose + 2 NAD+ + H2O recombinant forms Ugd(BCAL2946) and Ugd(BCAM0855) have similar in vitro Ugd activity Burkholderia cenocepacia J2315 UDP-glucuronate + 2 NADH + 2 H+
-
?

Subunits

Subunits Comment Organism
homodimer recombinant form of Ugd(BCAL2946) Burkholderia cenocepacia J2315
homodimer recombinant form of Ugd(BCAM0855) Burkholderia cenocepacia J2315

Synonyms

Synonyms Comment Organism
UDP-glucose dehydrogenase
-
Burkholderia cenocepacia J2315
Ugd
-
Burkholderia cenocepacia J2315

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.127
-
UDP-glucose
-
Burkholderia cenocepacia J2315
0.133
-
UDP-glucose
-
Burkholderia cenocepacia J2315
0.154
-
NAD+
-
Burkholderia cenocepacia J2315
0.156
-
NAD+
-
Burkholderia cenocepacia J2315