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Literature summary for 1.1.1.22 extracted from

  • Easley, K.E.; Sommer, B.J.; Boanca, G.; Barycki, J.J.; Simpson, M.A.
    Characterization of human UDP-glucose dehydrogenase reveals critical catalytic roles for lysine 220 and aspartate 280 (2007), Biochemistry, 46, 369-378.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C276A site-directed mutagenesis, strong decrease in specific activity Homo sapiens
C276S site-directed mutagenesis, strong decrease in specific activity Homo sapiens
D280E site-directed mutagenesis, 3-fold increase in Km for UDP-glucose and a 2-fold reduced Vmax relative to that of the wild type Homo sapiens
D280N site-directed mutagenesis, putative active site residue, mutation severly impairs enzyme function Homo sapiens
K220A site-directed mutagenesis, putative active site residue, mutation severly impairs enzyme function Homo sapiens
K220H site-directed mutagenesis, putative active site residue, mutation severly impairs enzyme function Homo sapiens
K220R site-directed mutagenesis, putative active site residue, mutation severly impairs enzyme function Homo sapiens
K279A site-directed mutagenesis, strong decrease in specific activity Homo sapiens
K339A site-directed mutagenesis, 165fold decrease in affinity for UDP-glucose. Mutant forms a dimer, in contrast to hexameric wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0092
-
UDP-glucose wild-type, pH 7.4, 22°C Homo sapiens
0.022
-
UDP-glucose mutant K339A, pH 7.4, 22°C Homo sapiens
0.42
-
NAD+ wild-type, pH 7.4, 22°C Homo sapiens
0.53
-
NAD+ mutant K339A, pH 7.4, 22°C Homo sapiens
1.5
-
UDP-glucose mutant K339A, pH 7.4, 22°C Homo sapiens
2.1
-
NAD+ mutant K339A, pH 7.4, 22°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000031
-
mutant D280N, pH 7.4, 22°C Homo sapiens
0.00022
-
mutant K276A, pH 7.4, 22°C Homo sapiens
0.0004
-
mutant K220H, pH 7.4, 22°C Homo sapiens
0.00041
-
mutant K220R, pH 7.4, 22°C Homo sapiens
0.00247
-
mutant K279A, pH 7.4, 22°C Homo sapiens
0.0027
-
mutant K276S, pH 7.4, 22°C Homo sapiens
0.0029
-
mutant K220A, pH 7.4, 22°C Homo sapiens
0.41
-
mutant D280E, pH 7.4, 22°C Homo sapiens
0.69
-
wild-type, 22°C, pH 7.4 Homo sapiens
0.74
-
mutant K339A, pH 7.4, 22°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O
-
Homo sapiens UDP-glucuronate + 2 NADH + 2 H+
-
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