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Literature summary for 1.1.1.219 extracted from

  • Xie, D.Y.; Jackson, L.A.; Cooper, J.D.; Ferreira, D.; Paiva, N.L.
    Molecular and biochemical analysis of two cDNA clones encoding dihydroflavonol-4-reductase from Medicago truncatula (2004), Plant Physiol., 134, 979-994.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.219

Cloned(Commentary)

Cloned (Comment) Organism
isozyme MtDFR1, from a young seed cDNA library, functional expression in Escherichia coli Medicago truncatula
isozyme MtDFR2, from a young seed cDNA library, functional expression in Escherichia coli Medicago truncatula

Protein Variants

Protein Variants Comment Organism
additional information overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor does not lead to increased anthocyanin production in the flowers Medicago truncatula
additional information overexpression of the isozyme in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV35S promotor leads to increased anthocyanin production in the flowers Medicago truncatula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Medicago truncatula key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago truncatula Q6TQT0 isozyme MtDFR2; cv. Jemalong, 2 isozymes MtDFR1 and MtDFR2
-
Medicago truncatula Q6TQT1 isozyme MtDFR1; cv. Jemalong, 2 isozymes MtDFR1 and MtDFR2
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Medicago truncatula
-
leaf
-
 Medicago truncatula
-
additional information developmental gene expression pattern Medicago truncatula
-
seed
-
 Medicago truncatula
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-2,3-dihydrokaempferol + NADPH
-
 Medicago truncatula cis-3,4-leucopelargonidin + NADP+
-
 ?
(+)-2,3-dihydrokaempferol + NADPH low activity Medicago truncatula cis-3,4-leucopelargonidin + NADP+
-
 ?
(+/-)-fustin + NADPH preference for (-)-isomer Medicago truncatula ?
-
 ?
(-)-fustin + NADPH stereospecific for (-)-isomer Medicago truncatula ?
-
 ?
2,3-dihydromyricetin + NADPH low activity Medicago truncatula cis-3,4-leucodelphinidin + NADP+
-
 ?
2,3-dihydroquercetin + NADPH i.e. (+)-taxifolin, stereospecific for (+)-isomer Medicago truncatula cis-3,4-leucocyanidin + NADP+
-
 ?
2,3-dihydrorobinetin + NADPH
-
 Medicago truncatula ?
-
 ?
additional information key enzyme in flux control in biosynthetic branched pathways leading to anthocyanins and condensed tannins Medicago truncatula ?
-
 ?
additional information stereospecific enzyme, no activity with substances lacking the the hydroxyl group at the 3-position or with a double bond present between C2 and C3, e.g. quercetin, apigenin, eriodictyol, and kaempferol Medicago truncatula ?
-
 ?

Synonyms

Synonyms Comment Organism
DFR
-
 Medicago truncatula
dihydroflavonol 4-reductase
-
 Medicago truncatula
dihydroflavonol-4-reductase
-
 Medicago truncatula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Medicago truncatula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Medicago truncatula
7
-
-
 Medicago truncatula

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Medicago truncatula