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Literature summary for 1.1.1.219 extracted from

  • Singh, S.; McCallum, J.; Gruber, M.Y.; Towers, G.H.N.; Muir, A.D.; Bohm, B.A.; Koupai-Abyazani, M.R.; Glass, A.D.M.
    Biosynthesis of flavan-3-ols by leaf extracts of Onobrychis viciifolia (1997), Phytochemistry, 44, 425-432.
No PubMed abstract available
Search for more literature for 1.1.1.219

Inhibitors

Inhibitors Comment Organism Structure
diethyldithiocarbamate 50% inhibition at 2 mM Onobrychis viciifolia
dithiothreitol 50% inhibition at 5 mM Onobrychis viciifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Onobrychis viciifolia biosynthesis of proanthocyanidin polymers (condensed tannins) ?
-
 ?

Organism

Organism UniProt Comment Textmining
Onobrychis viciifolia
-
 sainfoin, cv. Melrose
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Onobrychis viciifolia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 measurement of reductase activity during leaf development Onobrychis viciifolia

Storage Stability

Storage Stability Organism
5°C, stable for 36 h Onobrychis viciifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-dihydromyricetin + NADPH i.e. 5'-hydroxy-dihydroquercetin Onobrychis viciifolia cis-3,4-leucodelphinidin + NADP+ i.e. 5,7,4'-trihydroxyflavan-3,4-cis-diol ?
(+)-dihydromyricetin + NADPH i.e. 5'-hydroxy-dihydroquercetin Onobrychis viciifolia cis-3,4-leucodelphinidin + NADP+ configuration 2R,3S-trans-3S,4S-cis-leucodelphinidin ?
(+)-dihydroquercetin + NADPH
-
 Onobrychis viciifolia cis-3,4-leucocyanidin + NADP+ i.e. 5,7,3',4'-tetrahydroxyflavan-3,4-cis-diol, 2,3-trans-configuration retained ?
additional information biosynthesis of proanthocyanidin polymers (condensed tannins) Onobrychis viciifolia ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Onobrychis viciifolia

pH Range

pH Minimum pH Maximum Comment Organism
6.4 7.4
-
 Onobrychis viciifolia

Cofactor

Cofactor Comment Organism Structure
NADH reduced activity compared to NADPH Onobrychis viciifolia
NADPH
-
 Onobrychis viciifolia