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Literature summary for 1.1.1.218 extracted from

  • Walker, E.H.; French, C.E.; Rathbone, D.A.; Bruce, N.C.
    Mechanistic studies of morphine dehydrogenase and stabilization against covalent inactivation (2000), Biochem. J., 345, 687-692.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.218

Cloned(Commentary)

Cloned (Comment) Organism
gene encoding morphine dehydrogenase on natural plasmid, expressed in Escherichia coli Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
C80S change of cysteine to serine: significantly improved stability Pseudomonas putida
D47N residue modified by site-directed mutagenesis, resulting in substantial loss of activity Pseudomonas putida
K76M residue modified by site-directed mutagenesis, resulting in substantial loss of activity Pseudomonas putida
Y52F residue modified by site-directed mutagenesis, resulting in substantial loss of activity Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
additional information competitive inhibition: NADP+ with respect to NADPH, codeine with respect to codeinone Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 codeinone cosubstrate NADPH Pseudomonas putida
0.45
-
 codeine cosubstrate NADP+ Pseudomonas putida
0.54
-
 morphine cosubstrate NADP+ Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31990
-
 based on sequence data Pseudomonas putida
32000
-
 elecrospray mass spectrometer Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 M10
-
Pseudomonas putida M10
-
 M10
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
codeine + NAD(P)+ non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5 Pseudomonas putida codeinone + NAD(P)H morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0 ?
codeine + NAD(P)+ non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5 Pseudomonas putida M10 codeinone + NAD(P)H morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0 ?

Cofactor

Cofactor Comment Organism Structure
NAD(P)H enzyme specific for the proR hydrogen of NADPH Pseudomonas putida