General Stability | Organism |
---|---|
dithiothreitol stabilizes | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeine + NADP+ | Pseudomonas putida | codeine as the sole carbon and energy source | codeinone + NADPH | - |
? | |
codeine + NADP+ | Pseudomonas putida M10 | codeine as the sole carbon and energy source | codeinone + NADPH | - |
? | |
morphine + NADP+ | Pseudomonas putida | highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source | morphinone + NADPH | - |
? | |
morphine + NADP+ | Pseudomonas putida M10 | highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source | morphinone + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
M10 | - |
Pseudomonas putida M10 | - |
M10 | - |
Purification (Comment) | Organism |
---|---|
gel filtration, various chromatographic techniques | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.67 | - |
morphine, NADP+ | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeine + NAD(P)+ | - |
Pseudomonas putida | codeinone + NAD(P)H | - |
? | |
codeine + NAD(P)+ | - |
Pseudomonas putida M10 | codeinone + NAD(P)H | - |
? | |
codeine + NADP+ | codeine as the sole carbon and energy source | Pseudomonas putida | codeinone + NADPH | - |
? | |
codeine + NADP+ | codeine as the sole carbon and energy source | Pseudomonas putida M10 | codeinone + NADPH | - |
? | |
morphine + NADP+ | enzyme activity only with NADP+ | Pseudomonas putida | morphinone + NADPH + H+ | - |
? | |
morphine + NADP+ | enzyme activity only with NADP+ | Pseudomonas putida M10 | morphinone + NADPH + H+ | - |
? | |
morphine + NADP+ | highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source | Pseudomonas putida | morphinone + NADPH | - |
? | |
morphine + NADP+ | highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source | Pseudomonas putida M10 | morphinone + NADPH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme assay | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
with NADP+ as cofactor, in glycine-NaOH buffer, oxidizing the C-6 hydroxy group of morphine and codeine | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the Pseudomonas enzyme acts only with NADP+ | Pseudomonas putida |