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Literature summary for 1.1.1.218 extracted from

  • Bruce, N.C.; Wilmot, C.J.; Jordan, K.N.; Trebilcock, A.E.; Stephens, L.D.G.; Lowe, Ch.R.
    Microbial degradation of the morphine alkaloids: identification of morphinone as an intermediate in the metabolism of morphine by Pseudomonas putida M10 (1990), Arch. Microbiol., 154, 465-470.
    View publication on PubMed

General Stability

General Stability Organism
dithiothreitol stabilizes Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
codeine + NADP+ Pseudomonas putida codeine as the sole carbon and energy source codeinone + NADPH
-
?
codeine + NADP+ Pseudomonas putida M10 codeine as the sole carbon and energy source codeinone + NADPH
-
?
morphine + NADP+ Pseudomonas putida highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source morphinone + NADPH
-
?
morphine + NADP+ Pseudomonas putida M10 highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source morphinone + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
M10
-
Pseudomonas putida M10
-
M10
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, various chromatographic techniques Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
2.67
-
morphine, NADP+ Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
codeine + NAD(P)+
-
Pseudomonas putida codeinone + NAD(P)H
-
?
codeine + NAD(P)+
-
Pseudomonas putida M10 codeinone + NAD(P)H
-
?
codeine + NADP+ codeine as the sole carbon and energy source Pseudomonas putida codeinone + NADPH
-
?
codeine + NADP+ codeine as the sole carbon and energy source Pseudomonas putida M10 codeinone + NADPH
-
?
morphine + NADP+ enzyme activity only with NADP+ Pseudomonas putida morphinone + NADPH + H+
-
?
morphine + NADP+ enzyme activity only with NADP+ Pseudomonas putida M10 morphinone + NADPH + H+
-
?
morphine + NADP+ highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source Pseudomonas putida morphinone + NADPH
-
?
morphine + NADP+ highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source Pseudomonas putida M10 morphinone + NADPH
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
enzyme assay Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
with NADP+ as cofactor, in glycine-NaOH buffer, oxidizing the C-6 hydroxy group of morphine and codeine Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADP+ the Pseudomonas enzyme acts only with NADP+ Pseudomonas putida