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Literature summary for 1.1.1.213 extracted from

  • Okochi, M.; Nakagawa, I.; Kobayashi, T.; Hayashi, S.; Furusaki, S.; Honda, H.
    Enhanced activity of 3alpha-hydroxysteroid dehydrogenase by addition of the co-solvent 1-butyl-3-methylimidazolium (l)-lactate in aqueous phase of biphasic systems for reductive production of steroids (2006), J. Biotechnol., 128, 376-382.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1-butyl-3-methylimidazolium L-lactate enhanced activity of 3alpha-hydroxysteroid dehydrogenase by addition of the co-solvent to 50 mM Tris-HCl buffer in aqueous phase of biphasic systems for reductive production of steroids. In a coupled-enzyme system comprising HSDH and formate dehydrogenase, a twofold increase in production rate of androsterone is obtained when utilizing 1-butyl-3-methylimidazolium L-lactate with NADH regeneration, overview Comamonas testosteroni
1-ethyl-3-methylimidazolium trifluoromethanesulfonate addition of the co-solvent leads to slightly enhanced enzyme activity n an aqueous-organic solvent system with Tris-HCl buffer, overview Comamonas testosteroni
additional information poor activation by 1-butyl-3-methylimidazolium trifluoromethanesulfonate and 1-butyl-3-methylimidazolium tetrafluoroborate Comamonas testosteroni

Application

Application Comment Organism
synthesis the enzyme is useful in reductive production of steroids. In a coupled-enzyme system comprising HSDH and formate dehydrogenase, a twofold increase in production rate of androsterone is obtained when utilizing 1-butyl-3-methylimidazolium L-lactate with NADH regeneration Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics in absence and presence of co-solvents, overview Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
androsterone + NAD+ Comamonas testosteroni
-
5alpha-androstane-3,17-dione + NADH
-
r

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Comamonas testosteroni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
androsterone + NAD+
-
Comamonas testosteroni 5alpha-androstane-3,17-dione + NADH
-
r

Synonyms

Synonyms Comment Organism
HSDH
-
Comamonas testosteroni
More cf. EC 1.1.1.50 Comamonas testosteroni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Comamonas testosteroni
NADH
-
Comamonas testosteroni