Cloned (Comment) | Organism |
---|---|
stable expression of wild-type and mutant enzymes in HEK-293 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
R276E | site-directed mutagenesis, the mutant shows increased preference for the oxidation reaction compared to the wild-type enzyme | Rattus norvegicus |
R276G | site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme | Rattus norvegicus |
R276M | site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | equilibrium kinetics of wild-type and mutant enzymes | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NADPH | Rattus norvegicus | - |
5alpha-androstane-3alpha,17beta-diol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NADPH | - |
Rattus norvegicus | 5alpha-androstane-3alpha,17beta-diol + NADP+ | - |
? | |
5alpha-dihydrotestosterone + NADPH | i.e. DHT or 5alpha-androstane-17beta-ol-3-one, the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 | Rattus norvegicus | 5alpha-androstane-3alpha,17beta-diol + NADP+ | i.e. 5alpha-androstane-3alpha,17beta-diol | ? |
Synonyms | Comment | Organism |
---|---|---|
3alphaHSD | - |
Rattus norvegicus |
AKR1C9 | - |
Rattus norvegicus |
More | cf. EC 1.1.1.50 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | 1000fold lower activity with NADH compared to NADPH | Rattus norvegicus |