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Literature summary for 1.1.1.213 extracted from
- Kinetic and stereochemical characterization of hamster liver 3alpha-hydroxysteroid dehydrogenase and 3alpha(17beta)-hydroxysteroid dehydrogenase (1991), J. Biochem., 109, 770-775.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cibacron blue | nucleotide analog, competitive with respect to NADP+, noncompetitive to androsterone | Mesocricetus auratus |  |
| Hexestrol | hydroxysteroid analog, uncompetitive versus NADP+, competitive versus androsterone | Mesocricetus auratus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00008 | - |
5alpha-androstane-3,17-dione | - |
Mesocricetus auratus | |
| 0.00016 | - |
NADPH | - |
Mesocricetus auratus | |
| 0.0026 | - |
NADP+ | - |
Mesocricetus auratus | |
| 0.0094 | - |
3alpha-hydroxy-5alpha-androstan-17-one | - |
Mesocricetus auratus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mesocricetus auratus | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesocricetus auratus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | bi bi mechanism, NADP+ binds to the free enzyme before the hydroxysteroid substrate, the ketosteroid product leaves first, followed by NADPH | Mesocricetus auratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mesocricetus auratus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3alpha-hydroxy-5alpha-androstan-17-one + NADP+ | - |
Mesocricetus auratus | 5alpha-androstan-3,17-dione + NADPH | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mesocricetus auratus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
inactivated in absence of ligand, protected only by the addition of NADP+ | Mesocricetus auratus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | A-specific | Mesocricetus auratus | |
| NADPH | pro-R-hydrogen atom is transferred to the carbonyl substrate, A-specific | Mesocricetus auratus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00001 | - |
Cibacron blue | slope effect, 0.025 mM androsterone, varying NADP+ concentrations | Mesocricetus auratus | |
| 0.0002 | - |
Cibacron blue | slope effect, 0.25 mM NADP+, varying androsterone concentrations | Mesocricetus auratus | |
| 0.0005 | - |
Cibacron blue | intercept effect, 0.25 mM NADP+, varying androsterone concentrations | Mesocricetus auratus | |
| 0.0029 | - |
Hexestrol | slope effect, 0.25 mM NADP+, varying androsterone concentrations | Mesocricetus auratus | |
| 0.0031 | - |
Hexestrol | intercept effect, 0.025 mM androsterone, varying NADP+ concentrations | Mesocricetus auratus | |
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