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Literature summary for 1.1.1.21 extracted from

  • de Sousa, S.M.; Rosselli, L.K.; Kiyota, E.; da Silva, J.C.; Souza, G.H.; Peroni, L.A.; Stach-Machado, D.R.; Eberlin, M.N.; Souza, A.P.; Koch, K.E.; Arruda, P.; Torriani, I.L.; Yunes, J.A.
    Structural and kinetic characterization of a maize aldose reductase (2009), Plant Physiol. Biochem., 47, 98-104.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a recombinant His-tag fusion protein in Escherichia coli BL21(DE3) cells Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
DL-glyceraldehyde in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
108.1
-
D-ribose in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
179.5
-
D-xylitol in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
399.5
-
D-sorbitol in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
448.4
-
D-arabinose in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
466.8
-
D-xylose in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34560
-
calculated from amino acid sequence Zea mays
34560
-
recombinant enzyme, MALDI-TOF mass spectrometry Zea mays
34561
-
1 * 34561, MALDI-TOF mass spectrometry Zea mays
35000
-
SDS-PAGE Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays A2T1W7
-
-

Purification (Commentary)

Purification (Comment) Organism
agarose-IDA-Ni resin column chromatography, gel filtration Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
Zea mays
-
seed
-
Zea mays
-

Storage Stability

Storage Stability Organism
-20°C, the activity of the recombinant maize AKR4C7 enzyme is stable with no significant change in activity Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinose + NADPH + H+
-
Zea mays arabitol + NADP+
-
?
D-glucose + NADPH + H+ low affinity for D-glucose Zea mays D-sorbitol + NADP+
-
?
D-ribose + NADPH + H+
-
Zea mays D-ribitol + NADP+
-
?
D-xylose + NADPH + H+
-
Zea mays D-xylitol + NADP+
-
?
DL-glyceraldehyde + NADPH + H+
-
Zea mays glycerol + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 34561, MALDI-TOF mass spectrometry Zea mays

Synonyms

Synonyms Comment Organism
AKR4C7
-
Zea mays
aldose reductase
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Zea mays