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Literature summary for 1.1.1.206 extracted from

  • Zhao, K.; Zeng, J.; Zhao, T.; Zhang, H.; Qiu, F.; Yang, C.; Zeng, L.; Liu, X.; Chen, M.; Lan, X.; Liao, Z.
    Enhancing tropane alkaloid production based on the functional identification of tropine-forming reductase in Scopolia lurida, a tibetan medicinal plant (2017), Front. Plant Sci., 8, 1745 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Anisodus luridus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
40.9
-
 tropinone 30°C, pH 6.4 Anisodus luridus

Organism

Organism UniProt Comment Textmining
Anisodus luridus R4QQK2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root preferential expression in root Anisodus luridus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tropinone + NADPH + H+
-
 Anisodus luridus tropine + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE, recombinant protein Anisodus luridus

Synonyms

Synonyms Comment Organism
TRI
-
 Anisodus luridus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.98
-
 tropinone 30°C, pH 6.4 Anisodus luridus

General Information

General Information Comment Organism
physiological function in TRI-overexpressing root cultures, the hyoscyamine contents are 1.7- to 2.9fold higher than those in control Anisodus luridus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.024
-
 tropinone 30°C, pH 6.4 Anisodus luridus