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show all sequences of 1.1.1.206

Functional characterisation of a tropine-forming reductase gene from Brugmansia arborea, a woody plant species producing tropane alkaloids

Qiang, W.; Xia, K.; Zhang, Q.; Zeng, J.; Huang, Y.; Yang, C.; Chen, M.; Liu, X.; Lan, X.; Liao, Z.; Phytochemistry 127, 12-22 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene TRI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, functional recombinant overexpression of His-tagged enzyme in Escherichia coli strain Rosetta
Brugmansia arborea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Brugmansia arborea
additional information
-
additional information
Michaelis–Menten kinetics
Datura stramonium
0.34
-
Tropine
pH 9.6, 22°C
Datura stramonium
0.46
-
4-methylcyclohexanone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
0.56
-
Tropine
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
2.65
-
tropinone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
4.18
-
tropinone
pH 6.4, 22°C
Datura stramonium
5.17
-
3-quinuclidinone
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
-
Brugmansia arborea
29700
-
-
Brugmansia arborea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tropinone + NADPH + H+
Datura stramonium
-
tropine + NADP+
-
-
r
tropinone + NADPH + H+
Brugmansia arborea
-
tropine + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brugmansia arborea
A0A088MI02
formerly Datura arborea
-
Datura stramonium
P50162
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta by nickel affinity chromatography
Brugmansia arborea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Brugmansia arborea
-
additional information
enzyme BaTRI is expressed in all examined organs but is most abundant in secondary roots
Brugmansia arborea
-
root
-
Brugmansia arborea
-
stem
-
Brugmansia arborea
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-quinuclidinone + NADPH + H+
-
741124
Datura stramonium
1-azabicyclo[2.2.2]octan-3-ol + NADP+
-
-
-
r
3-quinuclidinone + NADPH + H+
-
741124
Brugmansia arborea
1-azabicyclo[2.2.2]octan-3-ol + NADP+
-
-
-
r
4-methylcyclohexanone + NADPH + H+
-
741124
Datura stramonium
4-methylcyclohexanol + NADP+
-
-
-
r
4-methylcyclohexanone + NADPH + H+
-
741124
Brugmansia arborea
4-methylcyclohexanol + NADP+
-
-
-
r
additional information
at pH 6.4, the non-ionizable substrate 4-methylcyclohexanone is uncharged, whereas the nitrogen-containing substrates tropinone and 3-quinuclidinone are positively charged. Enzyme BaTRI exhibits significantly higher apparent affinity for 4-methylcyclohexanone than tropinone and 3-quinuclidinone, of which only the deprotonated fractions can be bound by BaTRI
741124
Brugmansia arborea
?
-
-
-
-
tropine + NADP+
-
741124
Datura stramonium
tropinone + NADPH + H+
-
-
-
r
tropine + NADP+
-
741124
Brugmansia arborea
tropinone + NADPH + H+
-
-
-
r
tropinone + NADPH + H+
-
741124
Datura stramonium
tropine + NADP+
-
-
-
r
tropinone + NADPH + H+
-
741124
Brugmansia arborea
tropine + NADP+
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 29000, recombinant enzyme, SDS-PAGE, x * 29700, about, sequence calculation
Brugmansia arborea
More
enzyme three-dimensional structure molecular modeling, overview
Brugmansia arborea
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.54
-
3-quinuclidinone
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
2.4
-
tropinone
pH 6.4, 22°C
Datura stramonium
2.93
-
tropinone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
3.3
-
Tropine
pH 9.6, 22°C
Datura stramonium
4.67
-
4-methylcyclohexanone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
5.69
-
Tropine
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
8
tropinone reduction reaction
Brugmansia arborea
6.8
8
tropinone reduction reaction
Datura stramonium
9.6
-
tropine oxidation reaction
Brugmansia arborea
9.9
-
tropine oxidation reaction
Datura stramonium
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Brugmansia arborea
NADP+
-
Datura stramonium
NADPH
-
Brugmansia arborea
NADPH
-
Datura stramonium
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Brugmansia arborea
sequence calculation
-
5.79
Datura stramonium
sequence calculation
-
5.79
Cloned(Commentary) (protein specific)
Commentary
Organism
gene TRI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, functional recombinant overexpression of His-tagged enzyme in Escherichia coli strain Rosetta
Brugmansia arborea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Brugmansia arborea
NADP+
-
Datura stramonium
NADPH
-
Brugmansia arborea
NADPH
-
Datura stramonium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Brugmansia arborea
additional information
-
additional information
Michaelis–Menten kinetics
Datura stramonium
0.34
-
Tropine
pH 9.6, 22°C
Datura stramonium
0.46
-
4-methylcyclohexanone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
0.56
-
Tropine
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
2.65
-
tropinone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
4.18
-
tropinone
pH 6.4, 22°C
Datura stramonium
5.17
-
3-quinuclidinone
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
-
Brugmansia arborea
29700
-
-
Brugmansia arborea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tropinone + NADPH + H+
Datura stramonium
-
tropine + NADP+
-
-
r
tropinone + NADPH + H+
Brugmansia arborea
-
tropine + NADP+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta by nickel affinity chromatography
Brugmansia arborea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Brugmansia arborea
-
additional information
enzyme BaTRI is expressed in all examined organs but is most abundant in secondary roots
Brugmansia arborea
-
root
-
Brugmansia arborea
-
stem
-
Brugmansia arborea
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-quinuclidinone + NADPH + H+
-
741124
Datura stramonium
1-azabicyclo[2.2.2]octan-3-ol + NADP+
-
-
-
r
3-quinuclidinone + NADPH + H+
-
741124
Brugmansia arborea
1-azabicyclo[2.2.2]octan-3-ol + NADP+
-
-
-
r
4-methylcyclohexanone + NADPH + H+
-
741124
Datura stramonium
4-methylcyclohexanol + NADP+
-
-
-
r
4-methylcyclohexanone + NADPH + H+
-
741124
Brugmansia arborea
4-methylcyclohexanol + NADP+
-
-
-
r
additional information
at pH 6.4, the non-ionizable substrate 4-methylcyclohexanone is uncharged, whereas the nitrogen-containing substrates tropinone and 3-quinuclidinone are positively charged. Enzyme BaTRI exhibits significantly higher apparent affinity for 4-methylcyclohexanone than tropinone and 3-quinuclidinone, of which only the deprotonated fractions can be bound by BaTRI
741124
Brugmansia arborea
?
-
-
-
-
tropine + NADP+
-
741124
Datura stramonium
tropinone + NADPH + H+
-
-
-
r
tropine + NADP+
-
741124
Brugmansia arborea
tropinone + NADPH + H+
-
-
-
r
tropinone + NADPH + H+
-
741124
Datura stramonium
tropine + NADP+
-
-
-
r
tropinone + NADPH + H+
-
741124
Brugmansia arborea
tropine + NADP+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 29000, recombinant enzyme, SDS-PAGE, x * 29700, about, sequence calculation
Brugmansia arborea
More
enzyme three-dimensional structure molecular modeling, overview
Brugmansia arborea
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.54
-
3-quinuclidinone
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
2.4
-
tropinone
pH 6.4, 22°C
Datura stramonium
2.93
-
tropinone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
3.3
-
Tropine
pH 9.6, 22°C
Datura stramonium
4.67
-
4-methylcyclohexanone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
5.69
-
Tropine
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
8
tropinone reduction reaction
Brugmansia arborea
6.8
8
tropinone reduction reaction
Datura stramonium
9.6
-
tropine oxidation reaction
Brugmansia arborea
9.9
-
tropine oxidation reaction
Datura stramonium
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Brugmansia arborea
sequence calculation
-
5.79
Datura stramonium
sequence calculation
-
5.79
General Information
General Information
Commentary
Organism
evolution
enzyme TRI belongs to the family of short chain dehydrogenases/reductases (SDRs) that catalyse NAD(P)(H)-dependent redox reactions
Brugmansia arborea
evolution
enzyme TRI belongs to the family of short chain dehydrogenases/reductases (SDRs) that catalyse NAD(P)(H)-dependent redox reactions
Datura stramonium
metabolism
two tropinone reductases (TRs) constitute an important branch point in the tropanalkaloid biosynthetic pathway. Tropinone reductase I or tropine-forming reductase, EC 1.1.1.206, reduces tropinone to tropine during tropanalkaloid biosynthesis, whereas tropinone reductase II, EC 1.1.1.236, reduces tropinone to pseudotropine, diverging metabolic flux to nortropane calystegine A3. TRI activity controls metabolic flux towards hyoscyamine and downstream tropanalkaloids' biosynthesis
Brugmansia arborea
metabolism
two tropinone reductases (TRs) constitute an important branch point in the tropanalkaloid biosynthetic pathway. Tropinone reductase I or tropine-forming reductase, EC 1.1.1.206, reduces tropinone to tropine during tropanalkaloid biosynthesis, whereas tropinone reductase II, EC 1.1.1.236, reduces tropinone to pseudotropine, diverging metabolic flux to nortropane calystegine A3. TRI activity controls metabolic flux towards hyoscyamine and downstream tropanalkaloids' biosynthesis
Datura stramonium
additional information
enzyme three-dimensional structure molecular modeling, overview
Brugmansia arborea
General Information (protein specific)
General Information
Commentary
Organism
evolution
enzyme TRI belongs to the family of short chain dehydrogenases/reductases (SDRs) that catalyse NAD(P)(H)-dependent redox reactions
Brugmansia arborea
evolution
enzyme TRI belongs to the family of short chain dehydrogenases/reductases (SDRs) that catalyse NAD(P)(H)-dependent redox reactions
Datura stramonium
metabolism
two tropinone reductases (TRs) constitute an important branch point in the tropanalkaloid biosynthetic pathway. Tropinone reductase I or tropine-forming reductase, EC 1.1.1.206, reduces tropinone to tropine during tropanalkaloid biosynthesis, whereas tropinone reductase II, EC 1.1.1.236, reduces tropinone to pseudotropine, diverging metabolic flux to nortropane calystegine A3. TRI activity controls metabolic flux towards hyoscyamine and downstream tropanalkaloids' biosynthesis
Brugmansia arborea
metabolism
two tropinone reductases (TRs) constitute an important branch point in the tropanalkaloid biosynthetic pathway. Tropinone reductase I or tropine-forming reductase, EC 1.1.1.206, reduces tropinone to tropine during tropanalkaloid biosynthesis, whereas tropinone reductase II, EC 1.1.1.236, reduces tropinone to pseudotropine, diverging metabolic flux to nortropane calystegine A3. TRI activity controls metabolic flux towards hyoscyamine and downstream tropanalkaloids' biosynthesis
Datura stramonium
additional information
enzyme three-dimensional structure molecular modeling, overview
Brugmansia arborea
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.1045
-
3-quinuclidinone
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
0.574
-
tropinone
pH 6.4, 22°C
Datura stramonium
1.106
-
tropinone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
9.706
-
Tropine
pH 9.6, 22°C
Datura stramonium
10.15
-
4-methylcyclohexanone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
10.16
-
Tropine
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.1045
-
3-quinuclidinone
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
0.574
-
tropinone
pH 6.4, 22°C
Datura stramonium
1.106
-
tropinone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
9.706
-
Tropine
pH 9.6, 22°C
Datura stramonium
10.15
-
4-methylcyclohexanone
pH 6.4, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
10.16
-
Tropine
pH 9.6, 22°C, recombinant His-tagged enzyme
Brugmansia arborea
Other publictions for EC 1.1.1.206
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741221
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-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
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1
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741124
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Functional characterisation of ...
Brugmansia arborea, Datura stramonium
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12-22
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-
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1
-
-
-
-
8
-
-
2
2
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5
-
-
1
-
-
4
-
-
9
2
-
-
-
6
4
-
-
4
-
2
-
-
-
1
4
-
-
-
-
-
-
8
-
-
2
2
-
-
-
1
-
4
-
-
9
2
-
-
-
6
4
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5
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Gene
516
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-
-
1
-
-
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4
6
-
-
1
3
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4
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-
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2
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-
21
2
1
-
5
4
1
2
-
3
-
1
-
-
-
1
3
-
-
-
-
4
-
6
-
-
1
3
-
-
-
1
-
2
-
-
21
2
1
-
5
4
1
2
-
1
-
3
3
-
4
4
726209
Dehghan
An atypical pattern of accumul ...
Hyoscyamus muticus, Hyoscyamus senecionis
Plant Physiol. Biochem.
70C
188-194
2013
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
740901
Cheng
Functional characterization of ...
Dendrobium nobile
J. Plant Physiol.
170
958-964
2013
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
3
1
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
3
1
1
-
-
-
1
-
-
-
1
1
1
1
-
-
740981
Chen
Molecular cloning and characte ...
Dendrobium nobile
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40
1145-1154
2013
-
-
1
-
-
-
-
4
-
-
2
1
-
3
-
-
1
-
-
4
-
-
3
2
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
4
-
-
2
1
-
-
-
1
-
4
-
-
3
2
1
-
-
-
1
-
-
-
2
4
4
2
3
3
741278
Kushwaha
Tropine forming tropinone redu ...
Withania somnifera
PLoS ONE
8
e74777
2013
-
-
1
-
-
-
-
7
-
3
1
1
-
4
-
-
1
-
-
6
-
-
14
2
1
-
1
6
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
7
-
3
1
1
-
-
-
1
-
6
-
-
14
2
1
-
1
6
1
-
-
-
1
4
4
1
6
6
725946
Kai
Enhancing the production of tr ...
Anisodus acutangulus
Mol. Biosyst.
8
2883-2890
2012
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
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1
1
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1
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Two tropinone reductases with ...
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1
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Partial purification and prope ...
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4
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7
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1
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4
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