Literature summary for 1.1.1.205 extracted from

Fernandez-Justel, D.; Nunez, R.; Martin-Benito, J.; Jimeno, D.; Gonzalez-Lopez, A.; Soriano, E.M.; Revuelta, J.L.; Buey, R.M.
A nucleotide-dependent conformational switch controls the polymerization of human IMP dehydrogenases to modulate their catalytic activity (2019), J. Mol. Biol., 431, 956-969 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
ADP	-	Homo sapiens
AMP	-	Homo sapiens
additional information	ATP shows no significant effect on the catalytic activity of the enzyme	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
isoform IMPDH2 in complex with GTP, hanging drop vapor diffusion method, using 25% PEG-1500 and 0.1 M buffer MIB (malonic acid, imidazole and boric acid) at pH 9.0. Isoform IMPDH2 in complex with GDP, hanging drop vapor diffusion method, using 25% PEG-1500 and 0.1 M sodium citrate at pH 5.5, 0.2 M lithium sulfate and 15% (v/v) ethanol	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D226N	the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP	Homo sapiens
K238E	the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP	Homo sapiens
L227P	the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP	Homo sapiens
N198K	the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP	Homo sapiens
R224P	the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP	Homo sapiens
R231P	the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP	Homo sapiens
Y12A	the mutation abrogates polymerization of the enzyme. Mutant enzymes remain as monodisperse tetramers in solution	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
GDP	-	Homo sapiens
GTP	-	Homo sapiens
additional information	enzyme cytoophidia are catalytically active and resistant to GDP allosteric inhibition	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	1 mM used in assay conditions	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
IMP + NAD+ + H2O	Homo sapiens	-	XMP + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P12268	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293T cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
IMP + NAD+ + H2O	-	Homo sapiens	XMP + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
octamer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
IMPDH2	isoform	Homo sapiens
inosine 5'-monophosphate dehydrogenase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Homo sapiens

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Release 2023.1 (January 2023)