Cloned (Comment) | Organism |
---|---|
expression as fusion protein with green fluorescence protein, in HEK-293 cell, HeLa cell and COS cell, and expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D226N | mutant of isoform IMPDH1(546), and mutant of isoform IMPDH1(595), both are localized to cytosol and exhibit kinetic parameters identical to wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | retinal isoforms do not bind significant fractions of a random pool of oligonucleotides | Homo sapiens | |
NAD+ | at high concentration, inhibition of canonical isoform. No inhibition of retinal-specific isoforms | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
inosine 5'-phosphate | isoform IMPDH1(514), pH 8.0, 25°C | Homo sapiens | |
0.01 | - |
inosine 5'-phosphate | mutant D226N of isoform IMPDH1(595), pH 8.0, 25°C | Homo sapiens | |
0.011 | - |
inosine 5'-phosphate | isoform IMPDH1(595), pH 8.0, 25°C | Homo sapiens | |
0.011 | - |
inosine 5'-phosphate | mutant D226N of isoform IMPDH1(546), pH 8.0, 25°C | Homo sapiens | |
0.014 | - |
NAD+ | isoform IMPDH1(514), pH 8.0, 25°C | Homo sapiens | |
0.015 | - |
inosine 5'-phosphate | isoform IMPDH1(546), pH 8.0, 25°C | Homo sapiens | |
0.016 | - |
NAD+ | mutant D226N of isoform IMPDH1(595), pH 8.0, 25°C | Homo sapiens | |
0.022 | - |
NAD+ | isoform IMPDH1(546), pH 8.0, 25°C | Homo sapiens | |
0.024 | - |
NAD+ | mutant D226N of isoform IMPDH1(546), pH 8.0, 25°C | Homo sapiens | |
0.026 | - |
NAD+ | isoform IMPDH1(595), pH 8.0, 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
type 1 enzyme, retinal-specific isoforms IMPDH1(546) and IMPDH1(595) and canonical isoform IMPDH1(514) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
retina | retinal-specific isoforms IMPDH1(546) and IMPDH1(595) | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Homo sapiens | xanthosine 5'-phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the C-terminal extension unique to the retinal isoforms of IMPDH blocks the nucleic acid binding site | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
inosine 5'-phosphate | isoform IMPDH1(595), pH 8.0, 25°C | Homo sapiens | |
0.22 | - |
inosine 5'-phosphate | isoform IMPDH1(546), pH 8.0, 25°C | Homo sapiens | |
0.22 | - |
inosine 5'-phosphate | mutant D226N of isoform IMPDH1(595), pH 8.0, 25°C | Homo sapiens | |
0.24 | - |
inosine 5'-phosphate | mutant D226N of isoform IMPDH1(546), pH 8.0, 25°C | Homo sapiens | |
0.31 | - |
inosine 5'-phosphate | isoform IMPDH1(514), pH 8.0, 25°C | Homo sapiens |