Cloned (Comment) | Organism |
---|---|
expression of full-length type I isozyme and of the His-tagged type I isozyme subdomain in Escherichia coli | Homo sapiens |
expression of wild-type and mutant enzyme core domains in Escherichia coli | Tritrichomonas suis |
Protein Variants | Comment | Organism |
---|---|---|
K230E/R231E | site-directed mutagenesis, the exchange in the subdomain leads to 20fold reduced affinity for nucleic acids | Tritrichomonas suis |
R212E/R217E | site-directed mutagenesis, the exchange in the subdomain leads to 20fold reduced affinity for nucleic acids | Tritrichomonas suis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no enzyme inhibition by nucleic acids | Escherichia coli | |
additional information | no enzyme inhibition by nucleic acids | Homo sapiens | |
additional information | no enzyme inhibition by nucleic acids | Tritrichomonas suis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | nucleic acid binding kinetics | Escherichia coli | |
additional information | - |
additional information | nucleic acid binding kinetics, recombinant full-length type I isozyme and subdomain | Homo sapiens | |
additional information | - |
additional information | nucleic acid binding kinetics, recombinant wild-type and mutant enzyme core domains | Tritrichomonas suis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | Escherichia coli | rate-limiting step of the de novo guanine nucleotide biosynthesis | xanthosine 5'-phosphate + NADH | - |
? | |
inosine 5'-phosphate + NAD+ + H2O | Homo sapiens | rate-limiting step of the de novo guanine nucleotide biosynthesis | xanthosine 5'-phosphate + NADH | - |
? | |
inosine 5'-phosphate + NAD+ + H2O | Tritrichomonas suis | rate-limiting step of the de novo guanine nucleotide biosynthesis | xanthosine 5'-phosphate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
type I and type II isozymes | - |
Tritrichomonas suis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant full-length type I isozyme from Escherichia coli by two steps of affinity chromatography, recombinant His-tagged type I isozyme subdomain from Escherichia coli by metal affinity chromatography and ammonium sulfate fractionation | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Escherichia coli | xanthosine 5'-phosphate + NADH | - |
? | |
inosine 5'-phosphate + NAD+ + H2O | - |
Homo sapiens | xanthosine 5'-phosphate + NADH | - |
? | |
inosine 5'-phosphate + NAD+ + H2O | - |
Tritrichomonas suis | xanthosine 5'-phosphate + NADH | - |
? | |
inosine 5'-phosphate + NAD+ + H2O | rate-limiting step of the de novo guanine nucleotide biosynthesis | Escherichia coli | xanthosine 5'-phosphate + NADH | - |
? | |
inosine 5'-phosphate + NAD+ + H2O | rate-limiting step of the de novo guanine nucleotide biosynthesis | Homo sapiens | xanthosine 5'-phosphate + NADH | - |
? | |
inosine 5'-phosphate + NAD+ + H2O | rate-limiting step of the de novo guanine nucleotide biosynthesis | Tritrichomonas suis | xanthosine 5'-phosphate + NADH | - |
? | |
additional information | the enzyme binds both RNA and DNA in vitro and in vivo | Escherichia coli | ? | - |
? | |
additional information | the enzyme binds both RNA and DNA in vitro and in vivo | Homo sapiens | ? | - |
? | |
additional information | the enzyme binds both RNA and DNA in vitro and in vivo | Tritrichomonas suis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Escherichia coli |
tetramer | - |
Homo sapiens |
tetramer | the enzyme contains a cystathione beta-synthase-like subdomain which is involved in nucleic acid binding | Tritrichomonas suis |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Escherichia coli |
IMPDH | - |
Homo sapiens |
IMPDH | - |
Tritrichomonas suis |
inosine 5'-monophosphate dehydrogenase | - |
Escherichia coli |
inosine 5'-monophosphate dehydrogenase | - |
Homo sapiens |
inosine 5'-monophosphate dehydrogenase | - |
Tritrichomonas suis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Tritrichomonas suis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Tritrichomonas suis |