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Literature summary for 1.1.1.205 extracted from

  • McLean, J.E.; Hamaguchi, N.; Belenky, P.; Mortimer, S.E.; Stanton, M.; Hedstrom, L.
    Inosine 5'-monophosphate dehydrogenase binds nucleic acids in vitro and in vivo (2004), Biochem. J., 379, 243-251.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length type I isozyme and of the His-tagged type I isozyme subdomain in Escherichia coli Homo sapiens
expression of wild-type and mutant enzyme core domains in Escherichia coli Tritrichomonas suis

Protein Variants

Protein Variants Comment Organism
K230E/R231E site-directed mutagenesis, the exchange in the subdomain leads to 20fold reduced affinity for nucleic acids Tritrichomonas suis
R212E/R217E site-directed mutagenesis, the exchange in the subdomain leads to 20fold reduced affinity for nucleic acids Tritrichomonas suis

Inhibitors

Inhibitors Comment Organism Structure
additional information no enzyme inhibition by nucleic acids Escherichia coli
additional information no enzyme inhibition by nucleic acids Homo sapiens
additional information no enzyme inhibition by nucleic acids Tritrichomonas suis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information nucleic acid binding kinetics Escherichia coli
additional information
-
additional information nucleic acid binding kinetics, recombinant full-length type I isozyme and subdomain Homo sapiens
additional information
-
additional information nucleic acid binding kinetics, recombinant wild-type and mutant enzyme core domains Tritrichomonas suis

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O Escherichia coli rate-limiting step of the de novo guanine nucleotide biosynthesis xanthosine 5'-phosphate + NADH
-
?
inosine 5'-phosphate + NAD+ + H2O Homo sapiens rate-limiting step of the de novo guanine nucleotide biosynthesis xanthosine 5'-phosphate + NADH
-
?
inosine 5'-phosphate + NAD+ + H2O Tritrichomonas suis rate-limiting step of the de novo guanine nucleotide biosynthesis xanthosine 5'-phosphate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
type I and type II isozymes
-
Tritrichomonas suis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant full-length type I isozyme from Escherichia coli by two steps of affinity chromatography, recombinant His-tagged type I isozyme subdomain from Escherichia coli by metal affinity chromatography and ammonium sulfate fractionation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O
-
Escherichia coli xanthosine 5'-phosphate + NADH
-
?
inosine 5'-phosphate + NAD+ + H2O
-
Homo sapiens xanthosine 5'-phosphate + NADH
-
?
inosine 5'-phosphate + NAD+ + H2O
-
Tritrichomonas suis xanthosine 5'-phosphate + NADH
-
?
inosine 5'-phosphate + NAD+ + H2O rate-limiting step of the de novo guanine nucleotide biosynthesis Escherichia coli xanthosine 5'-phosphate + NADH
-
?
inosine 5'-phosphate + NAD+ + H2O rate-limiting step of the de novo guanine nucleotide biosynthesis Homo sapiens xanthosine 5'-phosphate + NADH
-
?
inosine 5'-phosphate + NAD+ + H2O rate-limiting step of the de novo guanine nucleotide biosynthesis Tritrichomonas suis xanthosine 5'-phosphate + NADH
-
?
additional information the enzyme binds both RNA and DNA in vitro and in vivo Escherichia coli ?
-
?
additional information the enzyme binds both RNA and DNA in vitro and in vivo Homo sapiens ?
-
?
additional information the enzyme binds both RNA and DNA in vitro and in vivo Tritrichomonas suis ?
-
?

Subunits

Subunits Comment Organism
tetramer
-
Escherichia coli
tetramer
-
Homo sapiens
tetramer the enzyme contains a cystathione beta-synthase-like subdomain which is involved in nucleic acid binding Tritrichomonas suis

Synonyms

Synonyms Comment Organism
IMPDH
-
Escherichia coli
IMPDH
-
Homo sapiens
IMPDH
-
Tritrichomonas suis
inosine 5'-monophosphate dehydrogenase
-
Escherichia coli
inosine 5'-monophosphate dehydrogenase
-
Homo sapiens
inosine 5'-monophosphate dehydrogenase
-
Tritrichomonas suis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli
8
-
assay at Homo sapiens
8
-
assay at Tritrichomonas suis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NAD+
-
Homo sapiens
NAD+
-
Tritrichomonas suis