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Literature summary for 1.1.1.203 extracted from
- Characterization of a uronate dehydrogenase from Thermobispora bispora for production of glucaric acid from hemicellulose substrate (2018), World J. Microbiol. Biotechnol., 34, 102 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Thermobispora bispora |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is more active in PBS than in Tris-HCl buffer | Thermobispora bispora |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.115 | - |
NAD+ | at pH 7.0 and 60°C | Thermobispora bispora |  |
| 0.115 | - |
D-galacturonic acid | at pH 7.0 and 60°C | Thermobispora bispora | |
| 0.165 | - |
D-glucuronic acid from a beechwood xylan | at pH 7.0 and 60°C | Thermobispora bispora |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobispora bispora | D6Y7Y8 | - |
- |
| Thermobispora bispora DSM 43833 | D6Y7Y8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-affinity chromatography | Thermobispora bispora |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 63.4 | - |
with D-galaceturonic acid as substrate, at pH 7.0 and 60°C | Thermobispora bispora |
| 95.17 | - |
with D-glucuronic acid from a beechwood xylan as substrate, at pH 7.0 and 60°C | Thermobispora bispora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galacturonic acid + NAD+ | - |
Thermobispora bispora | D-galactaric acid + NADH + H+ | - |
? | |
| D-galacturonic acid + NAD+ | - |
Thermobispora bispora DSM 43833 | D-galactaric acid + NADH + H+ | - |
? | |
| D-glucuronic acid from a beechwood xylan + NAD+ | after 60 min of incubation at 50°C, the enzyme exhibits a conversion ratio for glucuronic acid to the glucaric acid of 84% on chemical reagent and 81.3% on hydrolysates from beechwood xylans formed by xylanase and alpha-glucuronidase | Thermobispora bispora | D-glucaric acid + NADH + H+ | - |
? | |
| D-glucuronic acid from a beechwood xylan + NAD+ | after 60 min of incubation at 50°C, the enzyme exhibits a conversion ratio for glucuronic acid to the glucaric acid of 84% on chemical reagent and 81.3% on hydrolysates from beechwood xylans formed by xylanase and alpha-glucuronidase | Thermobispora bispora DSM 43833 | D-glucaric acid + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Thermobispora bispora |
| ? | x * 30700, calculated from amino acid sequence | Thermobispora bispora |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Udh | - |
Thermobispora bispora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermobispora bispora |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 65 | about 45% activity at 45°C, about 60% activity at 50°C, 100% activity at 60°C, about 90% activity at 65°C, about 20% activity at 70°C | Thermobispora bispora |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | the purified enzyme retains over 58% of its activity after holding a pH ranging from 7.0 to 7.5 for 1 h at 60°C. After incubation at 65°C for 1 h, the activity of the enzyme is 6.8% of the original activity | Thermobispora bispora |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 58.333 | - |
D-galacturonic acid | at pH 7.0 and 60°C | Thermobispora bispora | |
| 65.882 | - |
D-glucuronic acid from a beechwood xylan | at pH 7.0 and 60°C | Thermobispora bispora | |
| 74.667 | - |
NAD+ | at pH 7.0 and 60°C | Thermobispora bispora | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermobispora bispora |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | more than 40% activity between pH 6.0 and 8.5 | Thermobispora bispora |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | after incubation at pH 7.0 and 7.5 for 1 h, the enzyme activity is 98.4 and 96.6%, respectively, but its stability decreases significantly below pH 7.0 or above pH 7.5 | Thermobispora bispora |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Thermobispora bispora | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 400.014 | - |
D-glucuronic acid from a beechwood xylan | at pH 7.0 and 60°C | Thermobispora bispora | |
| 509.018 | - |
D-galacturonic acid | at pH 7.0 and 60°C | Thermobispora bispora | |
| 622.22 | - |
NAD+ | at pH 7.0 and 60°C | Thermobispora bispora | |
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