BRENDA - Enzyme Database show
show all sequences of 1.1.1.203

Expression profiling of genes involved in ascorbate biosynthesis and recycling during fleshy root development in radish

Xu, Y.; Zhu, X.; Chen, Y.; Gong, Y.; Liu, L.; Plant Physiol. Biochem. 70, 269-277 (2013)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Raphanus sativus
-
L-galactonate + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Raphanus sativus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
skin and flesh. In the skin, the expression of L-galacturonate acid dehydrogenase is correlated with ascorbate levels
Raphanus sativus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
743517
Raphanus sativus
L-galactonate + NADP+
-
-
-
r
D-galacturonate + NADPH + H+
-
743517
Raphanus sativus
L-galactono-1,4-lactone + NADP+
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Raphanus sativus
NADPH
-
Raphanus sativus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Raphanus sativus
NADPH
-
Raphanus sativus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Raphanus sativus
-
L-galactonate + NADP+
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
skin and flesh. In the skin, the expression of L-galacturonate acid dehydrogenase is correlated with ascorbate levels
Raphanus sativus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
743517
Raphanus sativus
L-galactonate + NADP+
-
-
-
r
D-galacturonate + NADPH + H+
-
743517
Raphanus sativus
L-galactono-1,4-lactone + NADP+
-
-
-
r
General Information
General Information
Commentary
Organism
metabolism
the enzyme is proposed to be part of the salvage pathway in ascorbate production as galacturonate acid reductase, GalUR, EC 1.1.1.203, converting D-galacturonate to L-galactono-1,4-lactone, ascorbate biosynthesis and recycling pathways in plants, overview
Raphanus sativus
physiological function
ascorbate accumulation is affected mainly by biosynthesis rather than recycling in radish root, and the L-galactose pathway may be the major biosynthetic route of ascorbate, and moreover, the salvage pathway may also contribute to ascorbate accumulation. Ascorbate level regulation involves the D-galacturonate reductase and GDP-D-mannose diphosphorylase
Raphanus sativus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is proposed to be part of the salvage pathway in ascorbate production as galacturonate acid reductase, GalUR, EC 1.1.1.203, converting D-galacturonate to L-galactono-1,4-lactone, ascorbate biosynthesis and recycling pathways in plants, overview
Raphanus sativus
physiological function
ascorbate accumulation is affected mainly by biosynthesis rather than recycling in radish root, and the L-galactose pathway may be the major biosynthetic route of ascorbate, and moreover, the salvage pathway may also contribute to ascorbate accumulation. Ascorbate level regulation involves the D-galacturonate reductase and GDP-D-mannose diphosphorylase
Raphanus sativus
Other publictions for EC 1.1.1.203
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740393
Wagschal
Biochemical characterization o ...
Chromohalobacter salexigens, Polaromonas naphthalenivorans, Pseudomonas fluorescens, Pseudomonas fluorescens Pf0-1, Pseudomonas mendocina, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000
Enzyme Microb. Technol.
69
62-68
2015
-
-
5
1
-
-
3
25
-
-
-
14
-
10
-
-
5
-
-
-
-
-
14
5
5
-
-
14
5
-
-
10
1
-
-
-
-
5
10
1
-
-
-
3
1
25
-
-
-
14
-
-
-
5
-
-
-
-
14
5
5
-
-
14
5
-
-
-
-
5
5
-
15
15
740959
Pick
Characterization of uronate de ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970, Fulvimarina pelagi, Oceanicola granulosus, Oceanicola granulosus DSM 15982, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000, Streptomyces viridochromogenes, Streptomyces viridochromogenes DSM 40736
Microb. Biotechnol.
8
633-643
2015
-
-
5
-
-
-
-
40
-
-
-
18
-
19
-
-
-
-
-
-
-
-
36
-
5
5
5
35
5
5
-
10
-
-
-
-
-
5
10
-
-
-
-
-
-
40
-
-
-
18
-
-
-
-
-
-
-
-
36
-
5
5
5
35
5
5
-
-
-
10
10
-
32
32
743517
Xu
Expression profiling of genes ...
Raphanus sativus
Plant Physiol. Biochem.
70
269-277
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
725435
Parkkinen
Crystal structure of uronate d ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
J. Biol. Chem.
286
27294-27300
2011
-
-
1
1
1
-
-
-
-
-
-
4
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695552
Moon
Enzymatic assay of D-glucurona ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
Anal. Biochem.
392
183-185
2009
-
1
1
-
-
-
-
-
-
-
-
-
-
21
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695783
Moon
Production of glucaric acid fr ...
Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
Appl. Environ. Microbiol.
75
589-595
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
698607
Yoon
Cloning and characterization o ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970, Pseudomonas putida KT2440, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
J. Bacteriol.
191
1565-1573
2009
-
-
3
-
-
-
-
9
-
-
3
-
-
17
-
-
3
-
-
-
-
-
10
3
-
-
-
6
3
-
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
9
-
-
3
-
-
-
-
3
-
-
-
-
10
3
-
-
-
6
3
-
-
-
-
-
-
-
-
-
286231
Wagner
Uronic acid dehydrogenase from ...
Pseudomonas syringae
Eur. J. Biochem.
61
589-596
1976
-
-
-
-
-
1
4
3
-
-
2
2
-
2
-
-
1
-
1
1
1
2
3
1
1
-
3
2
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
3
-
-
2
2
-
-
-
1
1
1
1
2
3
1
1
-
3
2
-
-
-
-
-
-
-
-
-
-
286230
Bateman
Purification and properties of ...
Pseudomonas syringae
Arch. Biochem. Biophys.
136
97-105
1970
-
-
-
-
-
1
1
3
-
-
-
2
-
2
-
-
1
-
-
1
1
1
2
-
-
-
2
-
1
-
2
1
-
-
-
-
-
-
1
-
-
1
-
1
-
3
-
-
-
2
-
-
-
1
-
1
1
1
2
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-