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Literature summary for 1.1.1.202 extracted from

  • Yun, J.; Yang, M.; Magocha, T.A.; Zhang, H.; Xue, Y.; Zhang, G.; Qi, X.; Sun, W.
    Production of 1,3-propanediol using a novel 1,3-propanediol dehydrogenase from isolated Clostridium butyricum and co-biotransformation of whole cells (2018), Biores. Technol., 247, 838-843 .
    View publication on PubMed
Search for more literature for 1.1.1.202

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Clostridium butyricum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 NADH with propionaldehyde as cosubstrate, at pH 9.0 and 37°C Clostridium butyricum
0.107
-
 NAD+ with propane-1,3-diol as cosubstrate, at pH 11.0 and 30°C Clostridium butyricum
0.36
-
 propionaldehyde at pH 9.0 and 37°C Clostridium butyricum
2.78
-
 Propane-1,3-diol at pH 11.0 and 30°C Clostridium butyricum

Organism

Organism UniProt Comment Textmining
Clostridium butyricum
-
-
-
Clostridium butyricum YJH-09
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Clostridium butyricum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 with propane-1,2-diol as substrate, at pH 11.0 and 30°C Clostridium butyricum
1.9
-
 with glycerol as substrate, at pH 11.0 and 30°C Clostridium butyricum
6.5
-
 with acetone as substrate, at pH 9.0 and 37°C Clostridium butyricum
7.8
-
 with hydroxyacetone as substrate, at pH 9.0 and 37°C Clostridium butyricum
7.9
-
 with propane-1,3-diol as substrate, at pH 11.0 and 30°C Clostridium butyricum
25.7
-
 with propionaldehyde as substrate, at pH 9.0 and 37°C Clostridium butyricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanal + NADH + H+
-
 Clostridium butyricum propane-1,3-diol + NAD+
-
 r
3-hydroxypropanal + NADH + H+
-
 Clostridium butyricum YJH-09 propane-1,3-diol + NAD+
-
 r
acetone + NADH + H+ 25.3% activity compared to propionaldehyde Clostridium butyricum ?
-
 ?
glycerol + NAD+ 24.1% activity compared to propane-1,3-diol Clostridium butyricum dihydroxyacetone + NADH + H+
-
 r
glycerol + NAD+ 24.1% activity compared to propane-1,3-diol Clostridium butyricum YJH-09 dihydroxyacetone + NADH + H+
-
 r
hydroxyacetone + NADH + H+ 30.6% activity compared to propionaldehyde Clostridium butyricum ?
-
 ?
additional information the enzyme cannot oxidize 1-butanol, 1-propanol, and ethanol Clostridium butyricum ?
-
-
additional information the enzyme cannot oxidize 1-butanol, 1-propanol, and ethanol Clostridium butyricum YJH-09 ?
-
-
propane-1,2-diol + NAD+ 21.5% activity compared to propane-1,3-diol Clostridium butyricum 2-hydroxypropanal + NADH + H+
-
 r
propane-1,2-diol + NAD+ 21.5% activity compared to propane-1,3-diol Clostridium butyricum YJH-09 2-hydroxypropanal + NADH + H+
-
 r
propane-1,3-diol + NAD+ 100% activity Clostridium butyricum 3-hydroxypropanal + NADH + H+
-
 r
propane-1,3-diol + NAD+ 100% activity Clostridium butyricum YJH-09 3-hydroxypropanal + NADH + H+
-
 r
propionaldehyde + NADH + H+ 100% activity Clostridium butyricum propan-1-ol + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, recombinant enzyme, SDS-PAGE Clostridium butyricum
? x * 42000, calculated from amino acid sequence Clostridium butyricum

Synonyms

Synonyms Comment Organism
DhaT
-
 Clostridium butyricum
NADH-dependent 1,3-PD dehydrogenase
-
 Clostridium butyricum
PDOR
-
 Clostridium butyricum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 maximum oxidation activity Clostridium butyricum
37
-
 maximum reduction activity Clostridium butyricum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 maximum reduction activity Clostridium butyricum
11
-
 maximum oxidation activity Clostridium butyricum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Clostridium butyricum
NADH the enzyme prefers NADH to NAD+ as cofactor. 0.5 mM NADH is chosen as optimum concentration Clostridium butyricum