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Literature summary for 1.1.1.202 extracted from

  • Durgapal, M.; Kumar, V.; Yang, T.H.; Lee, H.J.; Seung, D.; Park, S.
    Production of 1,3-propanediol from glycerol using the newly isolated Klebsiella pneumoniae J2B (2014), Bioresour. Technol., 159, 223-231 .
    View publication on PubMed
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Application

Application Comment Organism
synthesis Escherichia coli is engineered to produce 1,3-propanediol from glycerol, an inexpensive carbon source. This is done by introducing a synthetic 1,3-propanediol production pathway in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli. When 10 mM succinate is added to the medium, the titer of 1,3-propanediol and the glycerol consumption increase to 9.9 and 23.84 g/l, respectively. In addition, the ratio of NADH to NAD+ increases by 43%. Succinate addition is a promising route for the biotechnological production of NADH-dependent microbial metabolites Klebsiella pneumoniae
synthesis NADH-dependent 1,3-propanediol oxidoreductase is a key enzyme for the production of 1,3-propanediol in soluble cell extract. Klebsiella pneumoniae J2B shows a high potential for the production of 1,3-propanediol from glycerol. Optimization of the culture conditions and the elimination of lactate synthesis improves 1,3-propanediol production significantly Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
a synthetic pathway 1,3-propanediol production pathway is introduced in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
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Klebsiella pneumoniae A7LGK0
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Klebsiella pneumoniae J2B
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanal + NADH + H+
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 Klebsiella pneumoniae propane-1,3-diol + NAD+
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 ?

Synonyms

Synonyms Comment Organism
DhaT
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 Klebsiella pneumoniae
NADH-dependent 1,3-propanediol oxidoreductase
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 Klebsiella pneumoniae
YqhD
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 Klebsiella pneumoniae