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Literature summary for 1.1.1.202 extracted from
- Overexpression and characterization of the gene encoding 1,3-propanediol oxidoreductase of Citrobacter freundii (2014), Key Eng. Mater., 636, 121-124.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene dhaT, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Citrobacter freundii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Citrobacter freundii | |
| 8.9 | - |
3-hydroxypropanal | pH and temperature not specified in the publication | Citrobacter freundii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
- |
Citrobacter freundii |
| 440000 | - |
recombinant His6-tagged enzyme, gel filtration | Citrobacter freundii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxypropanal + NADH + H+ | Citrobacter freundii | - |
propane-1,3-diol + NAD+ | - |
r | |
| propane-1,3-diol + NAD+ | Citrobacter freundii | - |
3-hydroxypropanal + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | P45513 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxypropanal + NADH + H+ | - |
Citrobacter freundii | propane-1,3-diol + NAD+ | - |
r | |
| propane-1,3-diol + NAD+ | - |
Citrobacter freundii | 3-hydroxypropanal + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodecamer | 10 * 42927, sequence calculation, 10 * 43000, recombinant His6-tagged enzyme, SDS-PAGE | Citrobacter freundii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,3-propanediol oxidoreductase | - |
Citrobacter freundii |
| DhaT | - |
Citrobacter freundii |
| PDOR | - |
Citrobacter freundii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | PDOR requires NAD(H) as a cofactor, but the highly conserved NAD(H) binding fingerprint pattern G-X-G-X-X-G is not present in the amino acid sequence | Citrobacter freundii | |
| NAD+ | - |
Citrobacter freundii | |
| NADH | - |
Citrobacter freundii | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzyme PDOR shows high similarity with member of the family of type III alcohol dehydrogenases. PDOR requires NAD(H) as a cofactor, but the highly conserved NAD(H) binding fingerprint pattern G-X-G-X-X-G is not present in the amino acid sequence. This is also characteristic of most type III alcohol dehydrogenases | Citrobacter freundii |
| metabolism | 1,3-propanediol oxidoreductase (PDOR) is the rate-limiting enzyme in 1,3-propandiol synthesis biological pathway. 3-Hydroxypropanal is an intermediary metabolite in the 1,3-propanediol synthesis pathway. It is also an inhibitor to the activity of glycerol dehydratase (GDHt) and PDOR. PDOR is the key rate-limiting enzyme of the 3-HPA transformation and 1,3-PD formation when high concentration of glycerol is used in fermentation | Citrobacter freundii |
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