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Literature summary for 1.1.1.202 extracted from

  • Lama, S.; Ro, S.; Seol, E.; Sekar, B.; Ainala, S.; Thangappan, J.; Song, H.; Seung, D.; Park, S.
    Characterization of 1,3-propanediol oxidoreductase (DhaT) from Klebsiella pneumoniae J2B (2015), Biotechnol. Bioprocess Eng., 20, 971-979.
No PubMed abstract available
Search for more literature for 1.1.1.202

Activating Compound

Activating Compound Comment Organism Structure
additional information poor effects by EDTA, 2-mercaptoethanol, and sodium carbazide Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in YqhD-deficient Escherichia coli strain BL21(DE3) Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Klebsiella pneumoniae
Co2+ 29% inhibition at 1 mM Klebsiella pneumoniae
Cu2+ 47% inhibition at 1 mM Klebsiella pneumoniae
Hg2+ complete inhibition at 1 mM Klebsiella pneumoniae
KCl 17% inhibition at 1 mM Klebsiella pneumoniae
additional information poor effects by LiCl, DTT and sodium bisulfite at 1 mM Klebsiella pneumoniae
Ni2+ 29% inhibition at 1 mM Klebsiella pneumoniae
Zn2+ 38% inhibition at 1 mM Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 NADH pH 7.0, 37°C Klebsiella pneumoniae
0.23
-
 NAD+ pH 9.0, 55°C Klebsiella pneumoniae
0.77
-
 3-hydroxypropanal pH 7.0, 37°C Klebsiella pneumoniae
0.94
-
 propionaldehyde pH 7.0, 37°C Klebsiella pneumoniae
2.16
-
 Valeraldehyde pH 7.0, 37°C Klebsiella pneumoniae
4.2
-
 Butyraldehyde pH 7.0, 37°C Klebsiella pneumoniae
4.25
-
 acetaldehyde pH 7.0, 37°C Klebsiella pneumoniae
7.44
-
 Propane-1,3-diol pH 9.0, 55°C Klebsiella pneumoniae
16.1
-
 formaldehyde pH 7.0, 37°C Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effects by NaCl, NH4Cl, and MgCl2 at 1 mM Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropanal + NADH + H+ Klebsiella pneumoniae under physiological conditions, DhaT mostly catalyzes the forward reaction propane-1,3-diol + NAD+
-
 r
3-hydroxypropanal + NADH + H+ Klebsiella pneumoniae J2B under physiological conditions, DhaT mostly catalyzes the forward reaction propane-1,3-diol + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae Q59477
-
-
Klebsiella pneumoniae J2B Q59477
-
-

Purification (Commentary)

Purification (Comment) Organism
soluble recombinant His-tagged enzyme 6.6-6.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.9
-
 crude cell extract with recombinant His-tagged enzyme, substrate propane-1,3-diol, pH 9.0, 55°C Klebsiella pneumoniae
13.1
-
 purified recombinant His-tagged enzyme, substrate propane-1,3-diol, pH 9.0, 55°C Klebsiella pneumoniae
13.7
-
 crude cell extract with recombinant His-tagged enzyme, substrate 3-hydroxypropanal, pH 7.0, 37°C Klebsiella pneumoniae
89.7
-
 purified recombinant His-tagged enzyme, substrate 3-hydroxypropanal, pH 7.0, 37°C Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanal + NADH + H+
-
 Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 r
3-hydroxypropanal + NADH + H+ under physiological conditions, DhaT mostly catalyzes the forward reaction Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 r
3-hydroxypropanal + NADH + H+
-
 Klebsiella pneumoniae J2B propane-1,3-diol + NAD+
-
 r
3-hydroxypropanal + NADH + H+ under physiological conditions, DhaT mostly catalyzes the forward reaction Klebsiella pneumoniae J2B propane-1,3-diol + NAD+
-
 r
acetaldehyde + NADH + H+
-
 Klebsiella pneumoniae ethanol + NAD+
-
 ?
acetaldehyde + NADH + H+
-
 Klebsiella pneumoniae J2B ethanol + NAD+
-
 ?
butyraldehyde + NADH + H+
-
 Klebsiella pneumoniae butan-1-ol + NAD+
-
 ?
formaldehyde + NADH + H+
-
 Klebsiella pneumoniae methanol + NAD+
-
 ?
formaldehyde + NADH + H+
-
 Klebsiella pneumoniae J2B methanol + NAD+
-
 ?
propane-1,3-diol + NAD+
-
 Klebsiella pneumoniae 3-hydroxypropanal + NADH + H+
-
 r
propane-1,3-diol + NAD+
-
 Klebsiella pneumoniae J2B 3-hydroxypropanal + NADH + H+
-
 r
propionaldehyde + NADH + H+
-
 Klebsiella pneumoniae propan-1-ol + NAD+
-
 ?
valeraldehyde + NADH + H+
-
 Klebsiella pneumoniae pentan-1-ol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
1,3-propanediol oxidoreductase
-
 Klebsiella pneumoniae
DhaT
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 aldehyde reduction Klebsiella pneumoniae
55
-
 alcohol oxidation Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 over 80% of maximal activity within this range, aldehyde reduction, profile overview Klebsiella pneumoniae
30 65 over 60% of maximal activity within this range, alcohol oxidation, profile overview Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 Valeraldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1
-
 propionaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.6
-
 formaldehyde pH 7.0, 37°C Klebsiella pneumoniae
5.2
-
 acetaldehyde pH 7.0, 37°C Klebsiella pneumoniae
8.7
-
 Butyraldehyde pH 7.0, 37°C Klebsiella pneumoniae
9.7
-
 NAD+ pH 9.0, 55°C Klebsiella pneumoniae
9.98
-
 Propane-1,3-diol pH 9.0, 55°C Klebsiella pneumoniae
59.4
-
 3-hydroxypropanal pH 7.0, 37°C Klebsiella pneumoniae
59.5
-
 NADH pH 7.0, 37°C Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 aldehyde reduction Klebsiella pneumoniae
9
-
 alcohol oxidation Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 activity range, aldehyde reduction, profile overview Klebsiella pneumoniae
8 10 activity range, alcohol oxidation, profile overview Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Klebsiella pneumoniae
NADH
-
 Klebsiella pneumoniae

General Information

General Information Comment Organism
additional information enzyme homology modeling and docking studies Klebsiella pneumoniae
physiological function Klebsiella pneumoniae converts 3 hydroxypropionaldehyde (3-HPA) to 1,3-propanediol (1,3-PD) during microbial production of 1,3-PD from glycerol Klebsiella pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 formaldehyde pH 7.0, 37°C Klebsiella pneumoniae
0.5
-
 Valeraldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1
-
 propionaldehyde pH 7.0, 37°C Klebsiella pneumoniae
1.1
-
 Propane-1,3-diol pH 9.0, 55°C Klebsiella pneumoniae
1.2
-
 acetaldehyde pH 7.0, 37°C Klebsiella pneumoniae
2.1
-
 Butyraldehyde pH 7.0, 37°C Klebsiella pneumoniae
42
-
 NAD+ pH 9.0, 55°C Klebsiella pneumoniae
76.6
-
 3-hydroxypropanal pH 7.0, 37°C Klebsiella pneumoniae
1723
-
 NADH pH 7.0, 37°C Klebsiella pneumoniae