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Literature summary for 1.1.1.202 extracted from

  • Chen, Z.; Liu, H.; Liu, D.
    Regulation of 3-hydroxypropionaldehyde accumulation in Klebsiella pneumoniae by overexpression of dhaT and dhaD genes (2009), Enzyme Microb. Technol., 45, 305-309.
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
glycerol
-
 Klebsiella pneumoniae
isopropyl-beta-D-thiogalactoside inducer of gene expression, results in the increased activity of PDOR in LB medium Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
the dhaD gene encoding glycerol dehydrogenase (GDH) and dhaT gene encoding 1,3-propanediol oxidoreductase (PDOR) inserted into pTD plasmid and overexpressed in Klebsiella pneumoniae ACCC 10082 Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
additional information in LB media, almost no activity of PDOR in Klebsiella pneumoniae ACCC10082, while it is higher in fermentation medium Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanal + NADH + H+ overexpression of PDOR increases activity by 3.2fold, enzyme activity ratio of PDOR/GDHt (glycerol dehydratase) also is increased. 3-hydroxypropanal accumulation is successfully decreased and the risk of fermentation cease is reduced at the same time by overexpression of PDOR and GDH (glycerol dehydrogenase) Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
1,3-propanediol oxidoreductase
-
 Klebsiella pneumoniae
PDOR
-
 Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NADH overexpression of PDOR leads to enhanced NADH and production ratio of NADH/NAD+ exceeds after the inducement of isopropyl-beta-D-thiogalactoside for the constructed strain Klebsiella pneumoniae