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Literature summary for 1.1.1.202 extracted from

  • Seo, M.Y.; Seo, J.W.; Heo, S.Y.; Baek, J.O.; Rairakhwada, D.; Oh, B.R.; Seo, P.S.; Choi, M.H.; Kim, C.H.
    Elimination of by-product formation during production of propane-1,3-diol in Klebsiella pneumoniae by inactivation of glycerol oxidative pathway (2009), Appl. Microbiol. Biotechnol., 84, 527-534.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
isopropyl-D-thiogalactoside DhaT activity in the AK strain harboring pBR-dhaT-orfWX remarkably increases in the presence of isopropyl-D-thiogalactoside, although the activity is not proportional to the concentration of the inducer Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
recombinant plasmids containing dhaT expressed in Klebsiella pneumoniae Cu, and the mutant strains AK and AR Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information Klebsiella pneumoniae mutant strain AK (dhaD and dhaK replaced with the lacZ promoter-apramycin resistance gene cassette, such that the lacZ promoter is inserted in front of dhaB) and mutant strain AR (deletion of the dhaR gene and replaced with the lacZ promoter-apramycin resistance gene cassette, defective in the oxidative branch and in the reductive branch due to the loss of the dhaT and orfWX). propane-1,3-diol is still produced at lower level in the mutant strains, even though dhaT is removed, thus a a putative oxidoreductase may catalyze the production of propane-1,3-diol at lower level compared to DhaT. Dramatic increase in the production of propane-1,3-diol in the recombinant strains harboring the plasmid pBR-dhaT or pBR-dhaT-orfWX, which contain dhaT. Amount of propane-1,3-diol produced is lower in the AK and AR strain, which is harboring pBR-dhaT-orfWX (5.2 g/l), than the corresponding Cu, which is harboring pBR-dhaT-orfWX (7.7 g/l). During fermentation, the production yield is higher in the AK strain (0.57 mol/mol) than the Cu strain (0.47 mol/mol) harboring pBR-dhaT-orfWX Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
 derivative of ATCC 200721
-
Klebsiella pneumoniae Cu
-
 derivative of ATCC 200721
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanal + NADH + H+
-
 Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 ?
3-hydroxypropanal + NADH + H+
-
 Klebsiella pneumoniae Cu propane-1,3-diol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
1,3-PD oxidoreductase
-
 Klebsiella pneumoniae
DhaT
-
 Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Klebsiella pneumoniae