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Literature summary for 1.1.1.201 extracted from

  • Wang, R.; Wu, J.; Jin, D.K.; Chen, Y.; Lv, Z.; Chen, Q.; Miao, Q.; Huo, X.; Wang, F.
    Structure of NADP+-bound 7beta-hydroxysteroid dehydrogenase reveals two cofactor-binding modes (2017), Acta Crystallogr. Sect. F, 73, 246-252 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Collinsella aerofaciens

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme bound to NADP+, sitting drop vapor diffusion method, using 0.1 M sodium malonate pH 7.0, 12% (w/v) polyethylene glycol 3350 Collinsella aerofaciens

Organism

Organism UniProt Comment Textmining
Collinsella aerofaciens
-
-
-
Collinsella aerofaciens ATCC 25986
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, Mono Q column chromatography, Mono S column chromatography and Superdex 75 gel filtration Collinsella aerofaciens

Synonyms

Synonyms Comment Organism
7beta-HSDH
-
 Collinsella aerofaciens

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Collinsella aerofaciens
NADPH dependent on Collinsella aerofaciens