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Literature summary for 1.1.1.2 extracted from

  • Asako, H.; Shimizu, M.; Itoh, N.
    Engineering of NADPH-dependent aldo-keto reductase from Penicillium citrinum by directed evolution to improve thermostability and enantioselectivity (2008), Appl. Microbiol. Biotechnol., 80, 805-812.
    View publication on PubMed
Search for more literature for 1.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Penicillium citrinum

Protein Variants

Protein Variants Comment Organism
K245R mutation leads to improved thermostability Penicillium citrinum
L54Q mutant retains 62% of its initial activity after heat treatment at 30°C for 6 h and mutation confers improved enantioselectivity Penicillium citrinum
L54Q/K245R/N271D mutant displays improved thermostability and mutation confers improved enantioselectivity Penicillium citrinum
L54Q/R104C mutation leads to improved thermostability and enantioselectivity Penicillium citrinum
N271D mutation leads to improved thermostability Penicillium citrinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 methyl 4-bromo-3-oxobutanoate wild-type, pH 6.5, 30°C Penicillium citrinum
1.2
-
 methyl 4-bromo-3-oxobutanoate mutant L54Q, pH 6.5, 30°C Penicillium citrinum

Organism

Organism UniProt Comment Textmining
Penicillium citrinum
-
-
-
Penicillium citrinum IFO 4631
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Penicillium citrinum ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Penicillium citrinum IFO 4631 ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
methyl 4-bromo-3-oxobutanoate + NADPH + H+
-
 Penicillium citrinum methyl (S)-4-bromo-3-hydroxybutanoate + NADP+
-
 ?
methyl 4-bromo-3-oxobutanoate + NADPH + H+
-
 Penicillium citrinum IFO 4631 methyl (S)-4-bromo-3-hydroxybutanoate + NADP+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 6 h, 15% residual activity for wild-type Penicillium citrinum
40
-
 2 h, remaining activity is 1.7% for wild-type, 3.8% for mutant K245R, 10.1% for mutant N271D, and 74.9% for mutant L54Q/R104C Penicillium citrinum
45
-
 7 h, complete loss of activity for wild-type, 69.9% residual activity for mutant L54Q/K245R/N271D, 18.1% for mutant L54Q/R104C/K245R/N271D Penicillium citrinum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Penicillium citrinum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1160
-
 methyl 4-bromo-3-oxobutanoate mutant L54Q, pH 6.5, 30°C Penicillium citrinum
1420
-
 methyl 4-bromo-3-oxobutanoate wild-type, pH 6.5, 30°C Penicillium citrinum