Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
NADPH | pH 7.0, 70°C | Pyrococcus furiosus | |
0.08 | - |
NADP+ | pH 10.0, 70°C | Pyrococcus furiosus | |
3.3 | - |
Pyruvaldehyde | pH 7.0, 70°C | Pyrococcus furiosus | |
60.8 | - |
2-Pentanol | pH 10.0, 70°C | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25962 | - |
2 * 25962, calculated from sequence | Pyrococcus furiosus |
26000 | - |
2 * 26000, SDS-PAGE | Pyrococcus furiosus |
50000 | 55000 | gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | O73949 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Storage Stability | Organism |
---|---|
4°C, several months, stable | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-butanol + NADP+ | the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol | Pyrococcus furiosus | 2-butanone + NADPH + H+ | - |
? | |
(S)-2-butanol + NADP+ | the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol | Pyrococcus furiosus | 2-butanone + NADPH + H+ | - |
? | |
2-pentanol + NADP+ | maximal activity in oxidative reaction | Pyrococcus furiosus | 2-pentanone + NADPH + H+ | - |
r | |
2-propanol + NADP+ | - |
Pyrococcus furiosus | acetone + NADPH + H+ | - |
? | |
additional information | a broad range of primary alcohols could be oxidized by AdhA and includes substrates with 2-10 carbon atoms. It shows activity towards a broad range of secondary alcohols that on average are oxidized faster than the primary alcohols. Alcohols with a second or a third hydroxyl group are converted less efficiently | Pyrococcus furiosus | ? | - |
? | |
pyruvaldehyde + NADPH + H+ | maximal activity in reductive reaction | Pyrococcus furiosus | lactaldehyde + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, SDS-PAGE | Pyrococcus furiosus |
dimer | 2 * 25962, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
AdhA | - |
Pyrococcus furiosus |
short-chain ADH | - |
Pyrococcus furiosus |
short-chain alcohol dehydrogenase | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrococcus furiosus |
90 | - |
- |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 150 h | Pyrococcus furiosus |
90 | - |
half-life: 22.5 h | Pyrococcus furiosus |
100 | - |
half-life: 25 min | Pyrococcus furiosus |
103 | - |
melting temperature is 102.7°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
aldehyde reduction | Pyrococcus furiosus |
10 | 11 | alcohol oxidation | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 9 | pH 6.7: about 80% of maximal activity, pH 9.0: about 80% of maximal activity | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Pyrococcus furiosus | |
NADPH | - |
Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-Pentanol | pH 10.0, 70°C | Pyrococcus furiosus | |
4.2 | - |
Pyruvaldehyde | pH 7.0, 70°C | Pyrococcus furiosus |