Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star, 27°C, Lb-medium, carbenicillin, kanamycin, plasmid pBtacLB-ADH + pBBR1MCS2fdh, 130rpm | Levilactobacillus brevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
coexpression of formate dehydrogenase and alcohol dehydrogenase reducing methyl acetoacetate to methyl (R)-3-hydroxy butanoate with NADH, photometrically at 340 nm in a mixture of 10mM methyl acetoacetate, 250 microM NADH, 1 mM MgCl2, in 100 mM potassium phosphate buffer, pH 6.0 | Levilactobacillus brevis |
1.3 | - |
coexpression of formate dehydrogenase and alcohol dehydrogenase, reducing methyl acetoacetate to methyl (R)-3-hydroxy butanoate with NADPH, photometrically at 340 nm in a mixture of 10 mM methyl acetoacetate, 250 microM NADPH, 1 mM MgCl2, in 100 mM potassium phosphate buffer, pH 6.0 | Levilactobacillus brevis |
6.5 | - |
coexpression of formate dehydrogenase and alcohol dehydrogenase, reducing methyl acetoacetate to methyl (R)-3-hydroxy butanoate with NADPH, photometrically at 340 nm in a mixture of 10 mM methyl acetoacetate, 250 microM NADPH, 1 mM MgCl2, in 100 mM potassium phosphate buffer, pH 6.0 | Levilactobacillus brevis |
8.1 | - |
cell-free protein | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl acetoacetate + NADH + H+ | - |
Levilactobacillus brevis | methyl (R)-3-hydroxy-butanoate + NAD+ | - |
? | |
methyl acetoacetate + NADPH + H+ | - |
Levilactobacillus brevis | methyl (R)-3-hydroxy-butanoate + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay pH | Levilactobacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | maximal activity with 0.7 micromol/min/mg | Levilactobacillus brevis | |
NADPH | maximal activity with 6.5 micromol/min/mg | Levilactobacillus brevis |