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Literature summary for 1.1.1.2 extracted from

  • Tani, A.; Sakai, Y.; Ishige, T.; Kato, N.
    Thermostable NADP(+)-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp. strain M-1: purification and characterization and gene expression in Escherichia coli (2000), Appl. Environ. Microbiol., 66, 5231-5235.
    View publication on PubMed
Search for more literature for 1.1.1.2

Activating Compound

Activating Compound Comment Organism Structure
hexadecane induces enzyme Acinetobacter sp.

Application

Application Comment Organism
synthesis develpoment of conversion processes for petrochemicals and oil-contaminated environments, cinnamyl aldehyde and cinnamyl alcohol used in flavor and perfume industry, anisaldehyde is used for perfume and toilet soaps, decylalcohol is used in the manufacture of plasticizers, a production system for this enzyme may be useful for industrial application as a biocatalyst in the future Acinetobacter sp.
synthesis reduction of industrially important compounds cinnamyl aldehyde and anisaldehyde, industrial bioconversion of useful alcohols and aldehydes Acinetobacter sp.

Cloned(Commentary)

Cloned (Comment) Organism
alrA gene PCR amplified, cloned and highly expressed in Escherichia coli JM109 Acinetobacter sp.

General Stability

General Stability Organism
unstable Acinetobacter calcoaceticus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 100% inhibition Acinetobacter sp.
Ca2+ 26% inhibition Acinetobacter sp.
Co2+ 91% inhibition Acinetobacter sp.
Hg2+ 100% inhibition Acinetobacter sp.
iodoacetamide
-
 Acinetobacter sp.
Mn2+ 29% inhibition Acinetobacter sp.
N-ethylmaleimide
-
 Acinetobacter sp.
Ni2+ 84% inhibition Acinetobacter sp.
p-mercuribenzoate
-
 Acinetobacter sp.
Zn2+ 100% inhibition Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40000, SDS-PAGE Acinetobacter sp.
160000
-
 gel filtration Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
 strainNCIB8250, strain HO1-N
-
Acinetobacter sp.
-
-
-
Acinetobacter sp. M-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter calcoaceticus
wild-type and recombinant protein Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 recombinant enzyme, expressed in Escherichia coli JM109 Acinetobacter sp.
560
-
-
 Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-hexadienal + NADPH
-
 Acinetobacter sp. 2,4-hexadien-1-ol + NADP+
-
 ?
2-decanone + NADPH
-
 Acinetobacter sp. 2-decanol + NADP+
-
 ?
2-methylundecanal + NADPH
-
 Acinetobacter sp. 2-methylundecanol + NADP+
-
 ?
3-heptanone + NADPH
-
 Acinetobacter sp. 3-heptanol + NADP+
-
 ?
3-octanone + NADPH
-
 Acinetobacter sp. 3-octanol + NADP+
-
 ?
acetaldehyde + NADPH + H+
-
 Acinetobacter sp. ethanol + NADP+
-
 ?
acetaldehyde + NADPH + H+
-
 Acinetobacter sp. M-1 ethanol + NADP+
-
 ?
anisaldehyde + NADPH + H+
-
 Acinetobacter sp. anisyl alcohol + NADP+
-
 r
benzaldehyde + NADPH
-
 Acinetobacter sp. benzyl alcohol + NADP+
-
 r
benzaldehyde + NADPH
-
 Acinetobacter sp. M-1 benzyl alcohol + NADP+
-
 r
hexadecanol + NADP+
-
 Acinetobacter calcoaceticus hexadecanal + NADPH
-
 r
hexanal + NADPH + H+
-
 Acinetobacter sp. hexanol + NADP+
-
 r
hexanal + NADPH + H+
-
 Acinetobacter sp. M-1 hexanol + NADP+
-
 r
m-fluorobenzaldehyde + NADPH + H+
-
 Acinetobacter sp. m-fluorobenzyl alcohol + NADP+
-
 ?
m-methylbenzaldehyde + NADPH
-
 Acinetobacter sp. m-methylbenzyl alcohol + NADP+
-
 ?
n-butyraldehyde + NADPH
-
 Acinetobacter sp. n-butanol + NADP+
-
 r
n-decanal + NADPH + H+
-
 Acinetobacter sp. n-decanol + NADP+
-
 r
n-dodecanal + NADPH + H+
-
 Acinetobacter sp. n-dodecanol + NADP+
-
 r
n-heptanal + NADPH
-
 Acinetobacter sp. heptanol + NADP+
-
 r
n-octanal + NADPH + H+
-
 Acinetobacter sp. n-octanol + NADP+
-
 r
nonanal + NADPH
-
 Acinetobacter sp. ? + NADP+
-
 ?
o-fluorobenzaldehyde + NADPH + H+
-
 Acinetobacter sp. o-fluorobenzyl alcohol + NADP+
-
 ?
o-methylbenzaldehyde + NADPH
-
 Acinetobacter sp. o-methylbenzyl alcohol + NADP+
-
 ?
p-fluorobenzaldehyde + NADPH + H+
-
 Acinetobacter sp. p-fluorobenzyl alcohol + NADP+
-
 ?
p-methylbenzaldehyde + NADPH
-
 Acinetobacter sp. p-methylbenzyl alcohol + NADP+
-
 ?
p-methylbenzaldehyde + NADPH
-
 Acinetobacter sp. M-1 p-methylbenzyl alcohol + NADP+
-
 ?
pentanal + NADPH
-
 Acinetobacter sp. pentanol + NADP+
-
 r
pentanal + NADPH
-
 Acinetobacter sp. M-1 pentanol + NADP+
-
 r
phenylacetaldehyde + NADPH + H+
-
 Acinetobacter sp. phenylethanol + NADP+
-
 r
propanal + NADPH + H+
-
 Acinetobacter sp. propanol + NADP+
-
 r
tetradecanal + NADPH
-
 Acinetobacter sp. tetradecanol
-
 r
trans-2-decenal + NADPH
-
 Acinetobacter sp. trans-2-decen-1-ol + NADP+
-
 ?
trans-cinnamaldehyde + NADPH
-
 Acinetobacter sp. cinnamyl alcohol + NADP+
-
 ?
tridecanal + NADPH
-
 Acinetobacter sp. tridecanol + NADP+
-
 r
undecanal + NADPH
-
 Acinetobacter sp. undecanol + NADP+
-
 ?
vitamin A aldehyde + NADPH
-
 Acinetobacter sp. ? + NADP+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Acinetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 retains 90% activity after 40 min Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 n-heptanal reduction Acinetobacter sp.
9.5
-
 n-heptanol oxidation Acinetobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 9
-
 Acinetobacter sp.