Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hexadecane | induces enzyme | Acinetobacter sp. |
Application | Comment | Organism |
---|---|---|
synthesis | develpoment of conversion processes for petrochemicals and oil-contaminated environments, cinnamyl aldehyde and cinnamyl alcohol used in flavor and perfume industry, anisaldehyde is used for perfume and toilet soaps, decylalcohol is used in the manufacture of plasticizers, a production system for this enzyme may be useful for industrial application as a biocatalyst in the future | Acinetobacter sp. |
synthesis | reduction of industrially important compounds cinnamyl aldehyde and anisaldehyde, industrial bioconversion of useful alcohols and aldehydes | Acinetobacter sp. |
Cloned (Comment) | Organism |
---|---|
alrA gene PCR amplified, cloned and highly expressed in Escherichia coli JM109 | Acinetobacter sp. |
General Stability | Organism |
---|---|
unstable | Acinetobacter calcoaceticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 100% inhibition | Acinetobacter sp. | |
Ca2+ | 26% inhibition | Acinetobacter sp. | |
Co2+ | 91% inhibition | Acinetobacter sp. | |
Hg2+ | 100% inhibition | Acinetobacter sp. | |
iodoacetamide | - |
Acinetobacter sp. | |
Mn2+ | 29% inhibition | Acinetobacter sp. | |
N-ethylmaleimide | - |
Acinetobacter sp. | |
Ni2+ | 84% inhibition | Acinetobacter sp. | |
p-mercuribenzoate | - |
Acinetobacter sp. | |
Zn2+ | 100% inhibition | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Acinetobacter sp. |
160000 | - |
gel filtration | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
strainNCIB8250, strain HO1-N | - |
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. M-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter calcoaceticus |
wild-type and recombinant protein | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
recombinant enzyme, expressed in Escherichia coli JM109 | Acinetobacter sp. |
560 | - |
- |
Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-hexadienal + NADPH | - |
Acinetobacter sp. | 2,4-hexadien-1-ol + NADP+ | - |
? | |
2-decanone + NADPH | - |
Acinetobacter sp. | 2-decanol + NADP+ | - |
? | |
2-methylundecanal + NADPH | - |
Acinetobacter sp. | 2-methylundecanol + NADP+ | - |
? | |
3-heptanone + NADPH | - |
Acinetobacter sp. | 3-heptanol + NADP+ | - |
? | |
3-octanone + NADPH | - |
Acinetobacter sp. | 3-octanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Acinetobacter sp. | ethanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Acinetobacter sp. M-1 | ethanol + NADP+ | - |
? | |
anisaldehyde + NADPH + H+ | - |
Acinetobacter sp. | anisyl alcohol + NADP+ | - |
r | |
benzaldehyde + NADPH | - |
Acinetobacter sp. | benzyl alcohol + NADP+ | - |
r | |
benzaldehyde + NADPH | - |
Acinetobacter sp. M-1 | benzyl alcohol + NADP+ | - |
r | |
hexadecanol + NADP+ | - |
Acinetobacter calcoaceticus | hexadecanal + NADPH | - |
r | |
hexanal + NADPH + H+ | - |
Acinetobacter sp. | hexanol + NADP+ | - |
r | |
hexanal + NADPH + H+ | - |
Acinetobacter sp. M-1 | hexanol + NADP+ | - |
r | |
m-fluorobenzaldehyde + NADPH + H+ | - |
Acinetobacter sp. | m-fluorobenzyl alcohol + NADP+ | - |
? | |
m-methylbenzaldehyde + NADPH | - |
Acinetobacter sp. | m-methylbenzyl alcohol + NADP+ | - |
? | |
n-butyraldehyde + NADPH | - |
Acinetobacter sp. | n-butanol + NADP+ | - |
r | |
n-decanal + NADPH + H+ | - |
Acinetobacter sp. | n-decanol + NADP+ | - |
r | |
n-dodecanal + NADPH + H+ | - |
Acinetobacter sp. | n-dodecanol + NADP+ | - |
r | |
n-heptanal + NADPH | - |
Acinetobacter sp. | heptanol + NADP+ | - |
r | |
n-octanal + NADPH + H+ | - |
Acinetobacter sp. | n-octanol + NADP+ | - |
r | |
nonanal + NADPH | - |
Acinetobacter sp. | ? + NADP+ | - |
? | |
o-fluorobenzaldehyde + NADPH + H+ | - |
Acinetobacter sp. | o-fluorobenzyl alcohol + NADP+ | - |
? | |
o-methylbenzaldehyde + NADPH | - |
Acinetobacter sp. | o-methylbenzyl alcohol + NADP+ | - |
? | |
p-fluorobenzaldehyde + NADPH + H+ | - |
Acinetobacter sp. | p-fluorobenzyl alcohol + NADP+ | - |
? | |
p-methylbenzaldehyde + NADPH | - |
Acinetobacter sp. | p-methylbenzyl alcohol + NADP+ | - |
? | |
pentanal + NADPH | - |
Acinetobacter sp. | pentanol + NADP+ | - |
r | |
pentanal + NADPH | - |
Acinetobacter sp. M-1 | pentanol + NADP+ | - |
r | |
phenylacetaldehyde + NADPH + H+ | - |
Acinetobacter sp. | phenylethanol + NADP+ | - |
r | |
phenylacetaldehyde + NADPH + H+ | - |
Acinetobacter sp. M-1 | phenylethanol + NADP+ | - |
r | |
propanal + NADPH + H+ | - |
Acinetobacter sp. | propanol + NADP+ | - |
r | |
tetradecanal + NADPH | - |
Acinetobacter sp. | tetradecanol | - |
r | |
trans-2-decenal + NADPH | - |
Acinetobacter sp. | trans-2-decen-1-ol + NADP+ | - |
? | |
trans-cinnamaldehyde + NADPH | - |
Acinetobacter sp. | cinnamyl alcohol + NADP+ | - |
? | |
tridecanal + NADPH | - |
Acinetobacter sp. | tridecanol + NADP+ | - |
r | |
undecanal + NADPH | - |
Acinetobacter sp. | undecanol + NADP+ | - |
? | |
vitamin A aldehyde + NADPH | - |
Acinetobacter sp. | ? + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Acinetobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
retains 90% activity after 40 min | Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
n-heptanal reduction | Acinetobacter sp. |
9.5 | - |
n-heptanol oxidation | Acinetobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Acinetobacter sp. |