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Literature summary for 1.1.1.2 extracted from

  • Bryant, F.O.; Wiegel, J.; Ljungdahl, L.G.
    Purification and properties of primary and secondary alcohol dehydrogenases from Thermoanaerobacter ethanolicus (1988), Appl. Environ. Microbiol., 2, 460-465.
No PubMed abstract available

Application

Application Comment Organism
synthesis
-
Saccharomyces cerevisiae
synthesis
-
Acinetobacter sp.
synthesis
-
Zymomonas mobilis
synthesis potential use for industrial production of ethanol by fermentation, thermophilic fermentations offer the potential to separate ethanol from continous cultures at process temperature and reduced pressure during growth Thermoanaerobacter ethanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
NADP+ secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
0.15
-
NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
0.35
-
NADP+ secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
0.36
-
NADPH secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
0.83
-
NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.5
-
NADPH primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
16.4
-
propan-2-ol secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
16.4
-
ethanol primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
44.8
-
acetaldehyde secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
47.1
-
n-butanol primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
49
-
ethanol secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
210
-
acetaldehyde primary alcohol dehydrogenase Thermoanaerobacter ethanolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus
44500
-
4 * 44500, primary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus
172000
-
secondary alcohol dehydrogenase, gel filtration Thermoanaerobacter ethanolicus
184000
-
primary alcohol dehydrogenase, gel filtration Thermoanaerobacter ethanolicus

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Clostridium beijerinckii
-
-
-
Escherichia coli
-
-
-
Geobacillus stearothermophilus
-
-
-
Saccharomyces cerevisiae
-
yeast
-
Thermoanaerobacter brockii
-
-
-
Thermoanaerobacter ethanolicus
-
strain ATCC 31550
-
Thermoanaerobacter thermohydrosulfuricus
-
-
-
Zymomonas mobilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1° Adh and 2°Adh Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol + NADP+
-
Thermoanaerobacter ethanolicus ? + NADPH
-
?
1,3-butanediol + NADP+
-
Thermoanaerobacter ethanolicus ? + NADPH
-
?
1,3-propanediol + NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus ? + NADPH
-
?
1-pentanol + NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus n-pentanal + NADPH
-
r
2,3-pentanedione + NADPH + H+ secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus ? + NADP+
-
?
2-butanol + NADP+
-
Thermoanaerobacter ethanolicus butan-2-one + NADPH
-
r
2-butanol + NADP+
-
Thermoanaerobacter brockii butan-2-one + NADPH
-
r
2-heptanol + NADP+
-
Thermoanaerobacter ethanolicus heptan-2-one + NADPH
-
r
2-methyl-1-propanol + NADP+
-
Thermoanaerobacter ethanolicus 2-methylpropanal + NADPH
-
?
2-pentanol + NADP+
-
Thermoanaerobacter ethanolicus pentan-2-one + NADPH
-
r
3-hexanol + NADP+
-
Thermoanaerobacter ethanolicus hexan-3-one + NADPH
-
r
acetaldehyde + NADPH + H+
-
Acinetobacter sp. ethanol + NADP+
-
?
acetaldehyde + NADPH + H+
-
Thermoanaerobacter ethanolicus ethanol + NADP+
-
?
acetone + NADPH
-
Thermoanaerobacter ethanolicus 2-propanol + NADP+
-
?
ethanol + NADP+
-
Escherichia coli acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Saccharomyces cerevisiae acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Geobacillus stearothermophilus acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Acinetobacter sp. acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Zymomonas mobilis acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Thermoanaerobacter thermohydrosulfuricus acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Clostridium beijerinckii acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Thermoanaerobacter ethanolicus acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Thermoanaerobacter brockii acetaldehyde + NADPH + H+
-
r
ethylene glycol + NADP+
-
Thermoanaerobacter ethanolicus ? + NADPH
-
?
n-butanol + NADP+
-
Zymomonas mobilis butyraldehyde + NADPH
-
r
n-butanol + NADP+
-
Thermoanaerobacter ethanolicus butyraldehyde + NADPH
-
r
n-heptanol + NADP+
-
Saccharomyces cerevisiae n-heptanal + NADPH
-
r
n-heptanol + NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus n-heptanal + NADPH
-
r
n-propanol + NADP+
-
Thermoanaerobacter ethanolicus propionaldehyde + NADPH
-
r
propan-2-ol + NADP+
-
Thermoanaerobacter ethanolicus propanone + NADPH
-
r
propionaldehyde + NADPH
-
Thermoanaerobacter ethanolicus propanol + NADP+
-
r

Subunits

Subunits Comment Organism
tetramer
-
Acinetobacter sp.
tetramer
-
Thermoanaerobacter brockii
tetramer 4 * 44500, primary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus
tetramer 4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
secondary alcohol dehydrogenase with 2-propanol as substrate Thermoanaerobacter ethanolicus
85
-
primary enzyme, 1-butanol as substrate Thermoanaerobacter ethanolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
-
Acinetobacter sp.
70
-
no loss of activity during incubation for 2 h Thermoanaerobacter ethanolicus
80
-
secondary alcohol dehydrogenase with 2-propanol as substrate Thermoanaerobacter ethanolicus
85
-
primary enzyme, 1-butanol as substrate Thermoanaerobacter ethanolicus