Application | Comment | Organism |
---|---|---|
synthesis | - |
Saccharomyces cerevisiae |
synthesis | - |
Acinetobacter sp. |
synthesis | - |
Zymomonas mobilis |
synthesis | potential use for industrial production of ethanol by fermentation, thermophilic fermentations offer the potential to separate ethanol from continous cultures at process temperature and reduced pressure during growth | Thermoanaerobacter ethanolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
NADP+ | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
0.15 | - |
NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
0.35 | - |
NADP+ | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
0.36 | - |
NADPH | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
0.83 | - |
NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.5 | - |
NADPH | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
16.4 | - |
propan-2-ol | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
16.4 | - |
ethanol | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
44.8 | - |
acetaldehyde | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
47.1 | - |
n-butanol | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
49 | - |
ethanol | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
210 | - |
acetaldehyde | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
44500 | - |
4 * 44500, primary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
172000 | - |
secondary alcohol dehydrogenase, gel filtration | Thermoanaerobacter ethanolicus |
184000 | - |
primary alcohol dehydrogenase, gel filtration | Thermoanaerobacter ethanolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Clostridium beijerinckii | - |
- |
- |
Escherichia coli | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Saccharomyces cerevisiae | - |
yeast | - |
Thermoanaerobacter brockii | - |
- |
- |
Thermoanaerobacter ethanolicus | - |
strain ATCC 31550 | - |
Thermoanaerobacter thermohydrosulfuricus | - |
- |
- |
Zymomonas mobilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1° Adh and 2°Adh | Thermoanaerobacter ethanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + NADP+ | - |
Thermoanaerobacter ethanolicus | ? + NADPH | - |
? | |
1,3-butanediol + NADP+ | - |
Thermoanaerobacter ethanolicus | ? + NADPH | - |
? | |
1,3-propanediol + NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | ? + NADPH | - |
? | |
1-pentanol + NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | n-pentanal + NADPH | - |
r | |
2,3-pentanedione + NADPH + H+ | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | ? + NADP+ | - |
? | |
2-butanol + NADP+ | - |
Thermoanaerobacter ethanolicus | butan-2-one + NADPH | - |
r | |
2-butanol + NADP+ | - |
Thermoanaerobacter brockii | butan-2-one + NADPH | - |
r | |
2-heptanol + NADP+ | - |
Thermoanaerobacter ethanolicus | heptan-2-one + NADPH | - |
r | |
2-methyl-1-propanol + NADP+ | - |
Thermoanaerobacter ethanolicus | 2-methylpropanal + NADPH | - |
? | |
2-pentanol + NADP+ | - |
Thermoanaerobacter ethanolicus | pentan-2-one + NADPH | - |
r | |
3-hexanol + NADP+ | - |
Thermoanaerobacter ethanolicus | hexan-3-one + NADPH | - |
r | |
acetaldehyde + NADPH + H+ | - |
Acinetobacter sp. | ethanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Thermoanaerobacter ethanolicus | ethanol + NADP+ | - |
? | |
acetone + NADPH | - |
Thermoanaerobacter ethanolicus | 2-propanol + NADP+ | - |
? | |
ethanol + NADP+ | - |
Escherichia coli | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Geobacillus stearothermophilus | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Acinetobacter sp. | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Zymomonas mobilis | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Thermoanaerobacter thermohydrosulfuricus | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Clostridium beijerinckii | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Thermoanaerobacter ethanolicus | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Thermoanaerobacter brockii | acetaldehyde + NADPH + H+ | - |
r | |
ethylene glycol + NADP+ | - |
Thermoanaerobacter ethanolicus | ? + NADPH | - |
? | |
n-butanol + NADP+ | - |
Zymomonas mobilis | butyraldehyde + NADPH | - |
r | |
n-butanol + NADP+ | - |
Thermoanaerobacter ethanolicus | butyraldehyde + NADPH | - |
r | |
n-heptanol + NADP+ | - |
Saccharomyces cerevisiae | n-heptanal + NADPH | - |
r | |
n-heptanol + NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | n-heptanal + NADPH | - |
r | |
n-propanol + NADP+ | - |
Thermoanaerobacter ethanolicus | propionaldehyde + NADPH | - |
r | |
propan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | propanone + NADPH | - |
r | |
propionaldehyde + NADPH | - |
Thermoanaerobacter ethanolicus | propanol + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Acinetobacter sp. |
tetramer | - |
Thermoanaerobacter brockii |
tetramer | 4 * 44500, primary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
tetramer | 4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
secondary alcohol dehydrogenase with 2-propanol as substrate | Thermoanaerobacter ethanolicus |
85 | - |
primary enzyme, 1-butanol as substrate | Thermoanaerobacter ethanolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Acinetobacter sp. |
70 | - |
no loss of activity during incubation for 2 h | Thermoanaerobacter ethanolicus |
80 | - |
secondary alcohol dehydrogenase with 2-propanol as substrate | Thermoanaerobacter ethanolicus |
85 | - |
primary enzyme, 1-butanol as substrate | Thermoanaerobacter ethanolicus |