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Literature summary for 1.1.1.199 extracted from

  • Estevez, M.P.; Legaz, E.; Olmeda, L.; Perez, F.J.; Vicente, C.
    Purification and properties of a new enzyme from Evernia prunastri, which reduces L-usnic acid (1981), Z. Naturforsch. C, 36, 35-39.
No PubMed abstract available
Search for more literature for 1.1.1.199

General Stability

General Stability Organism
thermolabile Evernia prunastri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
450000
-
 gel filtration Evernia prunastri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-usnic acid + NADH Evernia prunastri
-
 NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone
-
 ?

Organism

Organism UniProt Comment Textmining
Evernia prunastri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Evernia prunastri

Source Tissue

Source Tissue Comment Organism Textmining
thallus
-
 Evernia prunastri
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-usnic acid + NADH
-
 Evernia prunastri NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Evernia prunastri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 37
-
 Evernia prunastri
22
-
 about 30% of maximal activity Evernia prunastri
37
-
 about 35% of maximal activity Evernia prunastri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Evernia prunastri

pH Range

pH Minimum pH Maximum Comment Organism
6.8 9.2
-
 Evernia prunastri
6.8
-
 about 20% of maximal activity Evernia prunastri
9.2
-
 about 50% of maximal activity Evernia prunastri

Cofactor

Cofactor Comment Organism Structure
NADH strict requirement for NADH, not replaceable by NADPH Evernia prunastri