Application | Comment | Organism |
---|---|---|
synthesis | production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium | Pseudomonas sp. TCU-HL1 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. TCU-HL1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-Camphor | - |
Pseudomonas sp. TCU-HL1 | |
(-)-Camphor | - |
Pseudomonas sp. TCU-HL1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
(+)-borneol | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
0.09 | - |
(+)-borneol | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
0.09 | - |
(+)-borneol | soluble recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
0.12 | - |
(-)-borneol | refolded isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
0.12 | - |
(+)-borneol | refolded isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
0.12 | - |
(+)-borneol | refolded recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
0.21 | - |
(-)-borneol | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
1.5 | - |
(-)-borneol | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | Pseudomonas sp. TCU-HL1 | - |
(+)-camphor + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. TCU-HL1 | - |
- |
- |
Pseudomonas sp. TCU-HL1 | A0A1B3EB36 | isoform BDH1 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl Sepharose column chromatography and Q-Sepharose column chromatography | Pseudomonas sp. TCU-HL1 |
production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium | Pseudomonas sp. TCU-HL1 |
Renatured (Comment) | Organism |
---|---|
- |
Pseudomonas sp. TCU-HL1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (+)-camphor + NADH + H+ | - |
? | |
(+)-borneol + NAD+ | the enzyme prefers to degrade (+)-borneol, rather than (-)-borneol | Pseudomonas sp. TCU-HL1 | (+)-camphor + NADH + H+ | - |
? | |
(-)-borneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (-)-camphor + NADH + H+ | - |
? | |
additional information | enzyme additionally catalyzes the reaction with (-)-borneol, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate | Pseudomonas sp. TCU-HL1 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * about 30000, isoform BDH1, SDS-PAGE | Pseudomonas sp. TCU-HL1 |
? | x * about 35000, isoform BDH2, SDS-PAGE | Pseudomonas sp. TCU-HL1 |
Synonyms | Comment | Organism |
---|---|---|
BDH1 | - |
Pseudomonas sp. TCU-HL1 |
BDH1 | isoform | Pseudomonas sp. TCU-HL1 |
BDH2 | isoform | Pseudomonas sp. TCU-HL1 |
THL1_4180 | - |
Pseudomonas sp. TCU-HL1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
(-)-borneol | refolded isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
0.5 | - |
(+)-borneol | refolded isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
0.5 | - |
(+)-borneol | refolded recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
12.4 | - |
(-)-borneol | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
17 | - |
(+)-borneol | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
17 | - |
(+)-borneol | soluble recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
18.5 | - |
(-)-borneol | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
27.8 | - |
(+)-borneol | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. TCU-HL1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
(+)-Camphor | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
0.07 | - |
(+)-Camphor | soluble recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
0.08 | - |
(-)-Camphor | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
0.16 | - |
(+)-Camphor | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
0.16 | - |
(+)-Camphor | refolded recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
0.52 | - |
(-)-Camphor | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 |