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Literature summary for 1.1.1.198 extracted from

  • Khine, A.; Yang, M.; Hu, A.; Lin, G.; Toh, Y.; Chen, H.
    Production of optically pure (-)-borneol by Pseudomonas monteilii TCU-CK1 and characterization of borneol dehydrogenase involved (2020), Enzyme Microb. Technol., 139, 109586 .
    View publication on PubMed
Search for more literature for 1.1.1.198

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas monteilii

General Stability

General Stability Organism
The enzyme activity is significantly inhibited by phosphate buffer in the alkaline range Pseudomonas monteilii

Inhibitors

Inhibitors Comment Organism Structure
(+)-Camphor
-
 Pseudomonas monteilii
(-)-Camphor
-
 Pseudomonas monteilii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 (+)-borneol at pH 11.0 and 25°C Pseudomonas monteilii
0.13
-
 (+)-borneol pH 11, 25°C Pseudomonas monteilii
0.14
-
 (+)-borneol pH 7, 25°C Pseudomonas monteilii
0.14
-
 (+)-borneol at pH 7.0 and 25°C Pseudomonas monteilii
0.64
-
 (-)-borneol at pH 7.0 and 25°C Pseudomonas monteilii
0.85
-
 (-)-borneol at pH 11.0 and 25°C Pseudomonas monteilii

Organism

Organism UniProt Comment Textmining
Pseudomonas monteilii
-
-
-
Pseudomonas monteilii TCU-CK1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns Pseudomonas monteilii
phenyl-Sepharose column chromatography, Q-Sepharose column chromatography and MonoQ column chromatography Pseudomonas monteilii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-borneol + NAD+
-
 Pseudomonas monteilii (+)-camphor + NADH + H+
-
 ?
(+)-borneol + NAD+ (+)-borneol is the preferred substrate Pseudomonas monteilii (+)-camphor + NADH + H+
-
 ?
(+)-borneol + NAD+
-
 Pseudomonas monteilii TCU-CK1 (+)-camphor + NADH + H+
-
 ?
(+)-borneol + NAD+ (+)-borneol is the preferred substrate Pseudomonas monteilii TCU-CK1 (+)-camphor + NADH + H+
-
 ?
(-)-borneol + NAD+
-
 Pseudomonas monteilii (-)-camphor + NADH + H+
-
 ?
(-)-borneol + NAD+
-
 Pseudomonas monteilii TCU-CK1 (-)-camphor + NADH + H+
-
 ?
additional information (-)-borneol is an additional substrate, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate Pseudomonas monteilii ?
-
-
additional information the recombinant enzyme does not use isopropanol, cyclohexanol, cyclopentanol, or(+/-)-2-butanol as alternative substrates, and NADP+ as a cofactor Pseudomonas monteilii ?
-
-
additional information (-)-borneol is an additional substrate, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate Pseudomonas monteilii TCU-CK1 ?
-
-
additional information the recombinant enzyme does not use isopropanol, cyclohexanol, cyclopentanol, or(+/-)-2-butanol as alternative substrates, and NADP+ as a cofactor Pseudomonas monteilii TCU-CK1 ?
-
-

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Pseudomonas monteilii
? x * 27400, calculated from amino acid sequence Pseudomonas monteilii
? x * 30000, SDS-PAGE, x * 27400, calculated from sequence Pseudomonas monteilii

Synonyms

Synonyms Comment Organism
BDH
-
 Pseudomonas monteilii
BDH cf. EC 1.1.1.227 Pseudomonas monteilii
TCK1_3449
-
 Pseudomonas monteilii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.3
-
 (-)-borneol at pH 7.0 and 25°C Pseudomonas monteilii
29.4
-
 (-)-borneol at pH 11.0 and 25°C Pseudomonas monteilii
75
-
 (+)-borneol pH 7, 25°C Pseudomonas monteilii
75
-
 (+)-borneol at pH 7.0 and 25°C Pseudomonas monteilii
209
-
 (+)-borneol at pH 11.0 and 25°C Pseudomonas monteilii
209
-
 (+)-borneol pH 11, 25°C Pseudomonas monteilii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
 Pseudomonas monteilii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas monteilii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.29
-
 (+)-Camphor pH 7, 25°C Pseudomonas monteilii
0.29
-
 (+)-Camphor at pH 7.0 and 25°C Pseudomonas monteilii
0.98
-
 (+)-Camphor at pH 11.0 and 25°C Pseudomonas monteilii
0.98
-
 (+)-Camphor pH 11, 25°C Pseudomonas monteilii
1.03
-
 (-)-Camphor at pH 7.0 and 25°C Pseudomonas monteilii
1.07
-
 (-)-Camphor at pH 11.0 and 25°C Pseudomonas monteilii

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas monteilii calculated from amino acid sequence
-
 4.8
Pseudomonas monteilii calculated fromn sequence
-
 4.8

General Information

General Information Comment Organism
physiological function neither wild-tpe nor a gene deletion mutant is able to grow on (-)-borneol or (-)-camphor as sole carbon source. Both strains grow similarly on (+)-borneol or (+)-camphor Pseudomonas monteilii