Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. ATCC 17453 |
Protein Variants | Comment | Organism |
---|---|---|
E145G | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
E145L/Y188A | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
E145M/Y188A | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
E145M/Y188T | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
E145S/Y188A | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
E145S/Y188T | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
S145H | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
S145L | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
S145M | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
S145S | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
S146A | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
S146A/Y188A | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
S146A/Y188T | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
Y188A | mutant catalyzes stereoselective reduction of racemic camphor with 23% conversion and 99% enantiomeric excess | Pseudomonas putida |
Y188A | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
Y188I | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
Y188L | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
Y188T | mutant catalyzes stereoselective reduction of racemic camphor with 22% conversion and 99% enantiomeric excess | Pseudomonas putida |
Y188T | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
Y188V | the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme | Pseudomonas sp. ATCC 17453 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NAD+ | wild type enzyme, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.06 | - |
NAD+ | wild-type, pH 7.5, 30°C | Pseudomonas putida | |
0.095 | - |
(+)-borneol | wild-type, pH 7.5, 30°C | Pseudomonas putida | |
0.095 | - |
(+)-borneol | wild type enzyme, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.115 | - |
(-)-borneol | wild type enzyme, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.28 | - |
(+)-borneol | mutant enzyme S146A/Y188A, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.28 | - |
(+)-borneol | mutant Y188A, pH 7.5, 30°C | Pseudomonas putida | |
0.34 | - |
(-)-borneol | mutant enzyme S146A/Y188A, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.46 | - |
(+)-borneol | mutant enzyme S146A/Y188T, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.46 | - |
(+)-borneol | mutant Y188T, pH 7.5, 30°C | Pseudomonas putida | |
0.73 | - |
(-)-borneol | mutant enzyme S146A/Y188T, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | Pseudomonas sp. ATCC 17453 | - |
(+)-camphor + NADH + H+ | - |
r | |
(+)-camphor + NADH + H+ | Pseudomonas sp. ATCC 17453 | - |
(+)-borneol + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida ATCC 17453 | - |
- |
- |
Pseudomonas sp. ATCC 17453 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Pseudomonas sp. ATCC 17453 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | - |
Pseudomonas putida | (+)-camphor + NADH + H+ | - |
r | |
(+)-borneol + NAD+ | - |
Pseudomonas sp. ATCC 17453 | (+)-camphor + NADH + H+ | - |
r | |
(+)-borneol + NAD+ | - |
Pseudomonas putida ATCC 17453 | (+)-camphor + NADH + H+ | - |
r | |
(+)-camphor + NADH + H+ | - |
Pseudomonas putida | (+)-borneol + NAD+ | - |
r | |
(+)-camphor + NADH + H+ | - |
Pseudomonas sp. ATCC 17453 | (+)-borneol + NAD+ | - |
r | |
(+)-camphor + NADH + H+ | - |
Pseudomonas putida ATCC 17453 | (+)-borneol + NAD+ | - |
r | |
(+)-isoborneol + NAD+ | - |
Pseudomonas putida | (+)-isocamphor + NADH + H+ | - |
? | |
(+)-isoborneol + NAD+ | - |
Pseudomonas putida ATCC 17453 | (+)-isocamphor + NADH + H+ | - |
? | |
(+/-)-isoborneol + NADH + H+ | - |
Pseudomonas sp. ATCC 17453 | ? | - |
? | |
(-)-borneol + NAD+ | - |
Pseudomonas sp. ATCC 17453 | (-)-camphor + NADH + H+ | - |
? | |
(1R)-(+)-camphor + (1S)-(-)-camphor + NADH + H+ | - |
Pseudomonas sp. ATCC 17453 | (+)-borneol + (1S)-(-)-camphor + NAD+ | - |
? | |
additional information | enzyme also catalyzes the reaction of (-)-borneol and (-)-camphor, reaction of EC 1.1.1.227. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol | Pseudomonas putida | ? | - |
- |
|
additional information | isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, (+/-)-2-butanol, L-carveol, and DL-menthol are not accepted as substrates | Pseudomonas sp. ATCC 17453 | ? | - |
- |
|
additional information | enzyme also catalyzes the reaction of (-)-borneol and (-)-camphor, reaction of EC 1.1.1.227. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol | Pseudomonas putida ATCC 17453 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Pseudomonas sp. ATCC 17453 |
? | x * 27500, calculated from amino acid sequence | Pseudomonas sp. ATCC 17453 |
? | x * 27500, calculated from sequence, x * 31000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
BDH | - |
Pseudomonas sp. ATCC 17453 |
BDH1 | cf. EC 1.1.1.227 | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas putida |
30 | - |
- |
Pseudomonas sp. ATCC 17453 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NAD+ | at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.07 | - |
(-)-borneol | mutant enzyme S146A/Y188A, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.13 | - |
(-)-borneol | mutant enzyme S146A/Y188T, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.89 | - |
(+)-borneol | wild-type, pH 7.5, 30°C | Pseudomonas putida | |
0.89 | - |
(+)-borneol | at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
0.97 | - |
(-)-borneol | at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
1.51 | - |
(+)-borneol | mutant enzyme S146A/Y188A, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
1.51 | - |
(+)-borneol | mutant Y188A, pH 7.5, 30°C | Pseudomonas putida | |
3.32 | - |
(+)-borneol | mutant enzyme S146A/Y188T, at pH 7.5 and 30°C | Pseudomonas sp. ATCC 17453 | |
3.32 | - |
(+)-borneol | mutant Y188T, pH 7.5, 30°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
pH optimum for the reduction of camphor | Pseudomonas sp. ATCC 17453 |
4.5 | - |
reduction of camphor | Pseudomonas putida |
7.5 | - |
oxidation of borneol | Pseudomonas putida |
7.5 | - |
pH optimum for the oxidation of (+/-)-borneol | Pseudomonas sp. ATCC 17453 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. ATCC 17453 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas putida | calculated from sequence | - |
5.4 |