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Literature summary for 1.1.1.195 extracted from
- Molecular cloning and functional analysis of nine cinnamyl alcohol dehydrogenase family members in c (2014), Planta, 240, 1097-1112.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cad1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD1 in Escherichia coli strain M15 | Populus tomentosa |
| gene cad2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD1 in Escherichia coli strain M15 | Populus tomentosa |
| gene cad3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD3 in Escherichia coli strain M15 | Populus tomentosa |
| gene cad5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD5 in Escherichia coli strain M15 | Populus tomentosa |
| gene cad7, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD7 in Escherichia coli strain M15 | Populus tomentosa |
| gene cad9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD9 in Escherichia coli strain M15 | Populus tomentosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics | Populus tomentosa | |
| 0.0043 | - |
sinapyl aldehyde | pH 5.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa |  |
| 0.00985 | - |
sinapyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 0.01996 | - |
coniferyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 0.02062 | - |
coniferyl aldehyde | pH 5.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 0.146 | - |
coniferyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 1.969 | - |
sinapyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required | Populus tomentosa | |
| Zn2+ | required, motif for both Zn2+ binding, modelling | Populus tomentosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33460 | - |
- |
Populus tomentosa |
| 34000 | - |
- |
Populus tomentosa |
| 39050 | - |
- |
Populus tomentosa |
| 39350 | - |
- |
Populus tomentosa |
| 40000 | - |
- |
Populus tomentosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl aldehyde + NADPH + H+ | Populus tomentosa | - |
coniferyl alcohol + NADP+ | - |
r | |
| coniferyl aldehyde + NADPH + H+ | Populus tomentosa | low activity | coniferyl alcohol + NADP+ | - |
r | |
| sinapyl aldehyde + NADPH + H+ | Populus tomentosa | - |
sinapyl alcohol + NADP+ | - |
r | |
| sinapyl aldehyde + NADPH + H+ | Populus tomentosa | very low activity | sinapyl alcohol + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus tomentosa | A0A023RBJ1 | CAD7 | - |
| Populus tomentosa | KJ159967 | CAD12 | - |
| Populus tomentosa | T1WUT6 | CAD9 | - |
| Populus tomentosa | T1WUU2 | CAD2 | - |
| Populus tomentosa | T1WVG5 | CAD3 | - |
| Populus tomentosa | T1WW77 | CAD8 | - |
| Populus tomentosa | T1WW82 | CAD1 | - |
| Populus tomentosa | T1WWB9 | CAD5 | - |
| Populus tomentosa | T1WWR8 | CAD6 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged PtoCAD1 from Escherichia coli strain M15 by nickel affinity chromatography | Populus tomentosa |
| recombinant His-tagged PtoCAD2 from Escherichia coli strain M15 by nickel affinity chromatography | Populus tomentosa |
| recombinant His-tagged PtoCAD3 from Escherichia coli strain M15 by nickel affinity chromatography | Populus tomentosa |
| recombinant His-tagged PtoCAD5 from Escherichia coli strain M15 by nickel affinity chromatography | Populus tomentosa |
| recombinant His-tagged PtoCAD7 from Escherichia coli strain M15 by nickel affinity chromatography | Populus tomentosa |
| recombinant His-tagged PtoCAD9 from Escherichia coli strain M15 by nickel affinity chromatography | Populus tomentosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bud | - |
Populus tomentosa | - |
| bud | highest expression level in buds | Populus tomentosa | - |
| bud | very high expression level in buds | Populus tomentosa | - |
| additional information | expression analysis of CAD/CAD-like genes, overview | Populus tomentosa | - |
| additional information | expression analysis of CAD/CAD-like genes, overview. Moderate expression in all tissues of PtoCAD9, except for the very high expression in buds | Populus tomentosa | - |
| additional information | expression analysis of CAD/CAD-like genes, overview. No obvious expression variation in the expression of PtoCAD8, PtoCAD8 is moderately expressed in all tissues with no obvious preference | Populus tomentosa | - |
| additional information | expression analysis of CAD/CAD-like genes, overview. PtoCAD1 is the most abundantly expressed in each tissue | Populus tomentosa | - |
| additional information | expression analysis of CAD/CAD-like genes, overview. PtoCAD12 is moderately expressed in all tissues with no obvious preference | Populus tomentosa | - |
| additional information | expression analysis of CAD/CAD-like genes, overview. PtoCAD5 is expressed at low levels in all tissues | Populus tomentosa | - |
| additional information | expression analysis of CAD/CAD-like genes, overview. PtoCAD7 is expressed at low levels in all tissues | Populus tomentosa | - |
| additional information | no obvious expression variation in the expression of PtoCAD2 | Populus tomentosa | - |
| phloem | - |
Populus tomentosa | - |
| root | - |
Populus tomentosa | - |
| root | greatest expression in the root | Populus tomentosa | - |
| xylem | - |
Populus tomentosa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl aldehyde + NADPH + H+ | - |
Populus tomentosa | coniferyl alcohol + NADP+ | - |
r | |
| coniferyl aldehyde + NADPH + H+ | low activity | Populus tomentosa | coniferyl alcohol + NADP+ | - |
r | |
| additional information | PtoCAD3 has poor affinity for both substrates and is active only at high substrate concentrations | Populus tomentosa | ? | - |
? | |
| sinapyl aldehyde + NADPH + H+ | - |
Populus tomentosa | sinapyl alcohol + NADP+ | - |
r | |
| sinapyl aldehyde + NADPH + H+ | very low activity | Populus tomentosa | sinapyl alcohol + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, about, SDS-PAGE, x * 33460, sequence calculation | Populus tomentosa |
| ? | x * 40000, about, SDS-PAGE, x * 339420, sequence calculation | Populus tomentosa |
| ? | x * 40000, about, SDS-PAGE, x * 38540, sequence calculation | Populus tomentosa |
| ? | x * 40000, about, SDS-PAGE, x * 389400, sequence calculation | Populus tomentosa |
| ? | x * 40000, about, SDS-PAGE, x * 389700, sequence calculation | Populus tomentosa |
| ? | x * 40000, about, SDS-PAGE, x * 39050, sequence calculation | Populus tomentosa |
| ? | x * 40000, about, SDS-PAGE, x * 39270, sequence calculation | Populus tomentosa |
| ? | x * 40000, about, SDS-PAGE, x * 39290, sequence calculation | Populus tomentosa |
| ? | x * 40000, about, SDS-PAGE, x * 39350, sequence calculation | Populus tomentosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAD | - |
Populus tomentosa |
| CAD1 | - |
Populus tomentosa |
| CAD12 | - |
Populus tomentosa |
| CAD2 | - |
Populus tomentosa |
| CAD5 | - |
Populus tomentosa |
| CAD6 | - |
Populus tomentosa |
| CAD7 | - |
Populus tomentosa |
| CAD8 | - |
Populus tomentosa |
| CAD9 | - |
Populus tomentosa |
| cinnamyl alcohol dehydrogenase | - |
Populus tomentosa |
| cinnamyl alcohol dehydrogenase 1 | - |
Populus tomentosa |
| cinnamyl alcohol dehydrogenase 12 | - |
Populus tomentosa |
| cinnamyl alcohol dehydrogenase 2 | - |
Populus tomentosa |
| cinnamyl alcohol dehydrogenase 3 | - |
Populus tomentosa |
| cinnamyl alcohol dehydrogenase 5 | - |
Populus tomentosa |
| cinnamyl alcohol dehydrogenase 7 | - |
Populus tomentosa |
| cinnamyl alcohol dehydrogenase 9 | - |
Populus tomentosa |
| PtoCAD1 | - |
Populus tomentosa |
| PtoCAD12 | - |
Populus tomentosa |
| PtoCAD2 | - |
Populus tomentosa |
| PtoCAD3 | - |
Populus tomentosa |
| PtoCAD5 | - |
Populus tomentosa |
| PtoCAD6 | - |
Populus tomentosa |
| PtoCAD7 | - |
Populus tomentosa |
| PtoCAD8 | - |
Populus tomentosa |
| PtoCAD9 | - |
Populus tomentosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
aldehyde reduction | Populus tomentosa |
| 30 | - |
aldehyde reduction | Populus tomentosa |
| 50 | - |
aldehyde reduction | Populus tomentosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 50 | activity range, profile overview | Populus tomentosa |
| 10 | 55 | very high activity at 30-50°C, profile overview | Populus tomentosa |
| 10 | 45 | very high activity at 10-40°C, rapid decrease in activity above 40°C, profile overview | Populus tomentosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.63 | - |
sinapyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 7.78 | - |
coniferyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 8.68 | - |
coniferyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 10.09 | - |
sinapyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 16.59 | - |
sinapyl aldehyde | pH 5.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 45.42 | - |
coniferyl aldehyde | pH 5.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
aldehyde reduction | Populus tomentosa |
| 6 | - |
aldehyde reduction | Populus tomentosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 9 | profile overview | Populus tomentosa |
| 4 | 8.5 | profile overview | Populus tomentosa |
| 4.5 | 7.5 | activity range, profile overview | Populus tomentosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Populus tomentosa | |
| NADPH | - |
Populus tomentosa | |
| NADPH | motif for both NADPH binding modelling | Populus tomentosa | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Populus tomentosa | sequence calculation | - |
5.76 |
| Populus tomentosa | sequence calculation | - |
6.15 |
| Populus tomentosa | sequence calculation | - |
6.24 |
| Populus tomentosa | sequence calculation | - |
6.28 |
| Populus tomentosa | sequence calculation | - |
6.43 |
| Populus tomentosa | sequence calculation | - |
6.48 |
| Populus tomentosa | sequence calculation | - |
6.67 |
| Populus tomentosa | sequence calculation | - |
7.97 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | nine CAD/CAD-like genes in Populus tomentosa are classified into four classes based on expression patterns, phylogenetic analysis and biochemical properties | Populus tomentosa |
| evolution | nine CAD/CAD-like genes in Populus tomentosa are classified into four classes based on expression patterns, phylogenetic analysis and biochemical properties. Isozyme PtoCAD12 is the only protein in the group III, as it is distinct from other PtoCADs and closely related to PoptrCAD12, AtCAD1 and OsCAD1 | Populus tomentosa |
| additional information | enzyme structure modelling, overview. Residues T49, Q53, L58, M60, C95, W119, V276, P286, M289, L290, F299, and I300 are conserved and putatively involved in catalysis | Populus tomentosa |
| additional information | enzyme structure modelling, overview. Residues T49, Q53, L58, M60, C95, W119, V276, P286, M289, L290, F299, and I300 are conserved and putatively involved in catalysis, except for residue C58 in PtoCAD1 | Populus tomentosa |
| additional information | residues T49, Q53, L58, M60, C95, W119, V276, P286, M289, L290, F299, and I300 are conserved and putatively involved in catalysis | Populus tomentosa |
| physiological function | isozymes PtoCAD1, -2, and -8 function differently depending on the cellular environment | Populus tomentosa |
| physiological function | PtoCAD9 expression is very high in the bud, suggesting that it might be related to bud development | Populus tomentosa |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
sinapyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 59.4 | - |
coniferyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 390 | - |
coniferyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 1025 | - |
sinapyl aldehyde | pH 6.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 2202 | - |
coniferyl aldehyde | pH 5.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 3858 | - |
sinapyl aldehyde | pH 5.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
| 3859 | - |
sinapyl aldehyde | pH 5.0, 30°C, recombinant His-tagged enzyme | Populus tomentosa | |
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