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Literature summary for 1.1.1.192 extracted from

  • Nagashima, H.; Inoue, J.; Sasaki, E.; Yamamoto, S.; Sasaki, Y.; Yamauchi-Inomata, Y.; Harayama, S.
    Long-chain n-alkanol dehydrogenase from Pseudomonas putida (1996), J. Ferment. Bioeng., 82, 328-333.
No PubMed abstract available
Search for more literature for 1.1.1.192

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 n-butanol
-
 Pseudomonas putida
0.053
-
 n-Propanol
-
 Pseudomonas putida
0.095
-
 n-decanal
-
 Pseudomonas putida
0.14
-
 n-Hexanol
-
 Pseudomonas putida
0.17
-
 NADH
-
 Pseudomonas putida
0.18
-
 n-Pentanol
-
 Pseudomonas putida
0.23
-
 n-decanol
-
 Pseudomonas putida
0.26
-
 n-Heptanol
-
 Pseudomonas putida
0.31
-
 NAD+
-
 Pseudomonas putida
0.34
-
 n-Octanol
-
 Pseudomonas putida
0.36
-
 n-nonanol
-
 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc 1.1 mol per mol of subunit Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40000, SDS-PAGE Pseudomonas putida
163000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
long-chain alcohol + NAD+ + H2O long chain n-alcanols up to C12 Pseudomonas putida long-chain carboxylate + NADH
-
 r
n-butanol + NAD+ + H2O
-
 Pseudomonas putida n-butanal + NADH
-
 ?
n-decanol + NAD+ + H2O
-
 Pseudomonas putida n-decanal + NADH
-
 r
n-heptanol + NAD+ + H2O
-
 Pseudomonas putida n-heptanal + NADH
-
 ?
n-hexanol + NAD+ + H2O
-
 Pseudomonas putida n-hexanal + NADH
-
 ?
n-nonanol + NAD+ + H2O
-
 Pseudomonas putida n-nonanal + NADH
-
 ?
n-octanol + NAD+ + H2O
-
 Pseudomonas putida n-octanal + NADH
-
 ?
n-pentanol + NAD+ + H2O
-
 Pseudomonas putida n-pentanal + NADH
-
 ?
n-propanol + NAD+ + H2O
-
 Pseudomonas putida n-propanal + NADH
-
 ?

Subunits

Subunits Comment Organism
More N-terminal sequence Pseudomonas putida
tetramer 4 * 40000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Pseudomonas putida
70
-
 10 min, all activity lost Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 n-decanol, reduction of Pseudomonas putida
9.5
-
 n-decanol, oxidation of Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ absolute requirement Pseudomonas putida