Data extracted from this reference:
Inhibitors
1,10-phenanthroline
Amycolatopsis methanolica
CuCl2
Amycolatopsis methanolica
HgCl2
Amycolatopsis methanolica
KCN
Amycolatopsis methanolica
additional information
inhibition differs for ethanol and formaldehyde as substrates
Amycolatopsis methanolica
KM Value [mM]
1.2
1-Octanol
Amycolatopsis methanolica
1.7
12-Hydroxydodecanoic acid
Amycolatopsis methanolica
3.4
1-Hexanol
Amycolatopsis methanolica
26.5
1-Pentanol
Amycolatopsis methanolica
84
1-butanol
Amycolatopsis methanolica
324
1-propanol
Amycolatopsis methanolica
343
ethanol
Amycolatopsis methanolica
Metals/Ions
Zinc
6.1 atoms/enzyme molecule
Amycolatopsis methanolica
Molecular Weight [Da]
40000
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
117000
120000
PAGE, gel filtration
Amycolatopsis methanolica
Natural Substrates/ Products (Substrates)
long-chain alcohol + NAD+ + H2O
Amycolatopsis methanolica
enzyme also uses formaldehyde and short chain aliphatic alcohols
long-chain carboxylate + NADH
Organism
Amycolatopsis methanolica
Substrates and Products (Substrate)
12-hydroxydodecanoic acid + NAD+ + H2O
288324
Amycolatopsis methanolica
? + NADH
?
ethanol + NAD+ + H2O
288324
Amycolatopsis methanolica
ethanal + NADH
?
long-chain alcohol + NAD+ + H2O
enzyme also uses formaldehyde and short chain aliphatic alcohols
288324
Amycolatopsis methanolica
long-chain carboxylate + NADH
n-butanol + NAD+ + H2O
288324
Amycolatopsis methanolica
n-butanal + NADH
n-hexanol + NAD+ + H2O
288324
Amycolatopsis methanolica
n-hexanal + NADH
n-pentanol + NAD+ + H2O
288324
Amycolatopsis methanolica
n-pentanal + NADH
n-propanol + NAD+ + H2O
288324
Amycolatopsis methanolica
n-propanal + NADH
Subunits
More
N-terminal sequence
Amycolatopsis methanolica
trimer
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
Inhibitors (protein specific)
1,10-phenanthroline
Amycolatopsis methanolica
CuCl2
Amycolatopsis methanolica
HgCl2
Amycolatopsis methanolica
KCN
Amycolatopsis methanolica
additional information
inhibition differs for ethanol and formaldehyde as substrates
Amycolatopsis methanolica
KM Value [mM] (protein specific)
1.2
1-Octanol
Amycolatopsis methanolica
1.7
12-Hydroxydodecanoic acid
Amycolatopsis methanolica
3.4
1-Hexanol
Amycolatopsis methanolica
26.5
1-Pentanol
Amycolatopsis methanolica
84
1-butanol
Amycolatopsis methanolica
324
1-propanol
Amycolatopsis methanolica
343
ethanol
Amycolatopsis methanolica
Metals/Ions (protein specific)
Zinc
6.1 atoms/enzyme molecule
Amycolatopsis methanolica
Molecular Weight [Da] (protein specific)
40000
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
117000
120000
PAGE, gel filtration
Amycolatopsis methanolica
Natural Substrates/ Products (Substrates) (protein specific)
long-chain alcohol + NAD+ + H2O
Amycolatopsis methanolica
enzyme also uses formaldehyde and short chain aliphatic alcohols
long-chain carboxylate + NADH
Substrates and Products (Substrate) (protein specific)
12-hydroxydodecanoic acid + NAD+ + H2O
288324
Amycolatopsis methanolica
? + NADH
?
ethanol + NAD+ + H2O
288324
Amycolatopsis methanolica
ethanal + NADH
?
long-chain alcohol + NAD+ + H2O
enzyme also uses formaldehyde and short chain aliphatic alcohols
288324
Amycolatopsis methanolica
long-chain carboxylate + NADH
n-butanol + NAD+ + H2O
288324
Amycolatopsis methanolica
n-butanal + NADH
n-hexanol + NAD+ + H2O
288324
Amycolatopsis methanolica
n-hexanal + NADH
n-pentanol + NAD+ + H2O
288324
Amycolatopsis methanolica
n-pentanal + NADH
n-propanol + NAD+ + H2O
288324
Amycolatopsis methanolica
n-propanal + NADH
Subunits (protein specific)
More
N-terminal sequence
Amycolatopsis methanolica
trimer
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
Other publictions for EC 1.1.1.192
712962
Liu
Two novel metal-independent lo ...
Geobacillus thermodenitrificans, Geobacillus thermodenitrificans NG80-2
Microbiology
155
2078-2085
2009
1
-
1
-
-
-
10
5
-
3
4
-
-
4
-
-
-
-
-
-
-
-
26
1
1
-
1
3
1
1
-
4
-
-
-
1
-
2
6
-
-
-
-
19
-
5
-
5
4
-
-
-
-
-
-
-
-
-
26
1
2
-
2
3
2
2
-
-
-
-
-
-
5
5
656603
Luesch
Biosynthesis of 4-methylprolin ...
Nostoc sp.
J. Org. Chem.
68
83-91
2003
-
-
1
-
-
-
-
2
-
1
1
1
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
347799
Nagashima
-
Long-chain n-alkanol dehydroge ...
Pseudomonas putida
J. Ferment. Bioeng.
82
328-333
1996
-
-
-
-
-
-
-
11
-
1
2
-
-
1
-
-
1
-
-
-
-
-
9
2
1
-
2
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
1
2
-
-
-
-
1
-
-
-
-
9
2
1
-
2
-
2
-
-
-
-
-
-
-
-
-
288324
Van Ophem
NAD-linked, factor-dependent f ...
Amycolatopsis methanolica
Eur. J. Biochem.
206
511-518
1992
-
-
-
-
-
-
5
7
-
1
2
1
-
7
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
7
-
1
2
1
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347798
Ribas de Pouplana
-
Structural properties of long- ...
Equus caballus
Biochem. J.
276
433-438
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347795
Ueda
Long-chain alcohol dehydrogena ...
Candida tropicalis, Yarrowia lipolytica
Methods Enzymol.
188
171-175
1990
-
-
-
-
-
6
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
9
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
9
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
347796
Yamada
Subcellular localization of lo ...
Candida tropicalis
Arch. Microbiol.
128
145-151
1980
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347794
Lee
Characterization of fatty alco ...
Rattus norvegicus
J. Biol. Chem.
254
2892-2896
1979
1
-
-
-
-
-
3
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
2
-
-
1
-
-
-
1
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
347800
Eklund
Three-dimensional structure of ...
Equus caballus
J. Mol. Biol.
102
27-59
1976
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-