Protein Variants | Comment | Organism |
---|---|---|
P85W | mutagenesis to the corresponding residue in other mammalian AKR1C subfamily enzymes, results in broad substrate specificity for nonsteroidal carbonyls and alcohols | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzbromarone | - |
Mus musculus | |
estradiol | - |
Mus musculus | |
Ethacrynic acid | - |
Mus musculus | |
Flufenamic acid | - |
Mus musculus | |
additional information | less than 10% inhibition at 0.05 mM: phenolphthalein, hexestrol, medroxyprogesterone acetate, progesterone, testosterone, cortisol, dehydroepiandrosterone | Mus musculus | |
quercetin | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
NADPH | wild-type, pH 7, 37°C | Mus musculus | |
0.003 | - |
Prostaglandin D2 | wild-type, pH 7, 37°C | Mus musculus | |
0.03 | - |
9alpha,11beta-prostaglandin F2 | wild-type, pH 7, 37°C | Mus musculus | |
0.28 | - |
9alpha,11beta-prostaglandin F2 | mutant P85W, pH 7, 37°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9D5U2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | abundant expression | Mus musculus | - |
brain | low expression level | Mus musculus | - |
lung | low expression level | Mus musculus | - |
ovary | abundant expression | Mus musculus | - |
prostate gland | low expression level | Mus musculus | - |
small intestine | low expression level | Mus musculus | - |
testis | abundant expression | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9alpha,11beta-prostaglandin F2 + NADPH + H+ | - |
Mus musculus | prostaglandin D2 + NADP+ | - |
r | |
prostaglandin D2 + NADP+ | - |
Mus musculus | 9alpha,11beta-prostaglandin F2 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Akr1cl | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
9alpha,11beta-prostaglandin F2 | mutant P85W, pH 7, 37°C | Mus musculus | |
0.055 | - |
9alpha,11beta-prostaglandin F2 | wild-type, pH 7, 37°C | Mus musculus | |
0.093 | - |
Prostaglandin D2 | wild-type, pH 7, 37°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | prostaglandin D2 reductase activity | Mus musculus |
9.5 | - |
9alpha,11beta-prostaglandin F2 dehydrogenase activity | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0087 | - |
wild-type, pH 7, 37°C | Mus musculus | estradiol | |
0.014 | - |
wild-type, pH 7, 37°C | Mus musculus | quercetin | |
0.018 | - |
wild-type, pH 7, 37°C | Mus musculus | benzbromarone | |
0.037 | - |
wild-type, pH 7, 37°C | Mus musculus | Ethacrynic acid | |
0.054 | - |
wild-type, pH 7, 37°C | Mus musculus | Flufenamic acid |