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Literature summary for 1.1.1.188 extracted from

  • Suzuki, T.; Fujii, Y.; Miyano, M.; Chen, L.Y.; Takahashi, T.; Watanabe, K.
    cDNA cloning, expression, and mutagenesis study of liver-type prostaglandin F synthase (1999), J. Biol. Chem., 274, 241-248.
    View publication on PubMed
Search for more literature for 1.1.1.188

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cells Bos taurus

Protein Variants

Protein Variants Comment Organism
H170C fully active Bos taurus
K225S 2fold as active as wild-type Bos taurus
N306Y half as active as wild-type Bos taurus
R223L 5fold as active as wild-type Bos taurus
R27E fully active Bos taurus
R91Q fully active Bos taurus
S301R 2.5fold as active as wild-type Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 9,10-phenanthrenequinone native lung prostaglandin F synthase Bos taurus
0.0011
-
 9,10-phenanthrenequinone expressed liver prostaglandin F synthase Bos taurus
0.002
-
 9,10-phenanthrenequinone native liver prostaglandin F synthase Bos taurus
0.01
-
 prostaglandin H2 native lung prostaglandin F synthase Bos taurus
0.01
-
 Prostaglandin D2 native liver prostaglandin F synthase Bos taurus
0.015
-
 Prostaglandin D2 expressed liver prostaglandin F synthase Bos taurus
0.025
-
 prostaglandin H2 native and expressed liver prostaglandin F synthase Bos taurus
0.12
-
 Prostaglandin D2 native lung prostaglandin F synthase Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36740
-
 amino acid analysis, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 two isozymes, lung-type and liver-type
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-
lung
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
9,10-phenanthrenequinone + NADPH
-
 Bos taurus ?
-
 ?
prostaglandin H2 + NADPH + H+
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Bos taurus