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Literature summary for 1.1.1.184 extracted from
- A novel thermal stable carbonyl reductase from Bacillus cereus by gene mining as biocatalyst for beta-carbonyl ester asymmetric reduction reaction (2019), Catal. Lett., 149, 610-618 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus cereus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 80.2% residual activity in the presence of Ca2+ | Bacillus cereus |  |
| Co2+ | 70.7% residual activity in the presence of Co2+ | Bacillus cereus | |
| EDTA | 96.8% residual activity in the presence of EDTA | Bacillus cereus | |
| Mg2+ | 73.9% residual activity in the presence of Mg2+ | Bacillus cereus | |
| Mn2+ | 97.6% residual activity in the presence of Mn2+ | Bacillus cereus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.85 | - |
ethyl 4-chloroacetoacetate | at pH 7.0 and 37°C | Bacillus cereus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl 4-chloroacetoacetate + NADPH + H+ | - |
Bacillus cereus | ethyl S-4-chloro-3-hydroxybutyrate + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbonyl reductase 1 | - |
Bacillus cereus |
| CBR1 | - |
Bacillus cereus |
| NADPH-dependent carbonyl reductase | - |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
- |
Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | when incubated at 40°C and 50°C for 2 h, the enzyme maintains residual enzyme activity of more than 70% | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | more than 75% activity between pH 6.0 and 8.0 | Bacillus cereus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | dependent on | Bacillus cereus | |
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Release 2023.1 (January 2023)
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