Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.184 extracted from

  • Luo, W.; Du, H.; Bonku, E.; Hou, Y.; Li, L.; Wang, X.; Yang, Z.
    An alkali-tolerant carbonyl reductase from Bacillus subtilis by gene mining identification and application (2019), Catal. Lett., 149, 2973-2983 .
No PubMed abstract available
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 92.24% residual activity at 1 mM Bacillus subtilis
Mg2+ 94.22% residual activity at 1 mM Bacillus subtilis
additional information not inhibited by EDTA Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0539
-
 NADPH at pH 6.5 and 30°C Bacillus subtilis
1.855
-
 ethyl 4-chloroacetoacetate at pH 6.5 and 30°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 115.98% activity at 1 mM Bacillus subtilis
Cu2+ 114.16% activity at 1 mM Bacillus subtilis
Fe2+ 108.52% activity at 1 mM Bacillus subtilis
Mn2+ 114.16% activity at 1 mM Bacillus subtilis
additional information not affected by Li+, Fe3+ and Al3+ Bacillus subtilis
Ni2+ 106.9% activity at 1 mM Bacillus subtilis
Zn2+ 115.83% activity at 1 mM Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity resin column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fluoroacetophenone + NADPH + H+ 20.2% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis ? + NADP+
-
 ?
2'-fluoroacetophenone + NADPH + H+ 20.2% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis 168 ? + NADP+
-
 ?
2,2,2-trifluoroacetophenone + NADPH + H+ 126.2% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis (1S)-2,2,2-trifluoro-1-phenylethanol + NADP+
-
 ?
2,2,2-trifluoroacetophenone + NADPH + H+ 126.2% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis 168 (1S)-2,2,2-trifluoro-1-phenylethanol + NADP+
-
 ?
2-octanone + NADPH + H+ 64.6% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis ? + NADP+
-
 ?
2-octanone + NADPH + H+ 64.6% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis 168 ? + NADP+
-
 ?
3'-fluoroacetophenone + NADPH + H+ 18.8% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis ? + NADP+
-
 ?
3'-fluoroacetophenone + NADPH + H+ 18.8% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis 168 ? + NADP+
-
 ?
4'-fluoroacetophenone + NADPH + H+ 12.4% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis ? + NADP+
-
 ?
acetone + NADPH + H+ 20.2% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis propan-2-ol + NADP+
-
 ?
acetone + NADPH + H+ 20.2% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis 168 propan-2-ol + NADP+
-
 ?
acetophenone + NADPH + H+
-
 Bacillus subtilis (1S)-1-phenylethanol + NADP+
-
 ?
alpha-chloroacetophenone + NADPH + H+ 40.6% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis ? + NADP+
-
 ?
ethyl 4-chloroacetoacetate + NADPH + H+ 100% activity Bacillus subtilis ethyl S-4-chloro-3-hydroxybutyrate + NADP+
-
 ?
ethyl acetoacetate + NADPH + H+ 23.7% activity compared to ethyl 4-chloroacetoacetate Bacillus subtilis ethyl S-3-hydroxybutyrate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
YueD
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 55 the enzyme has half-lives of 18.57, 12.99, 0.76 and 0.06 h at 25, 35, 45 and 55°C respectively Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
 the enzyme keeps more than 70% of its peak activity after incubation in Tris-HCl buffer at pH 9.0 for 40 h Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Bacillus subtilis