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Literature summary for 1.1.1.184 extracted from

  • Li, Z.; Liu, W.; Chen, X.; Jia, S.; Wu, Q.; Zhu, D.; Ma, Y.
    Highly enantioselective double reduction of phenylglyoxal to (R)-1-phenyl-1,2-ethanediol by one NADPH-dependent yeast carbonyl reductase with a broad substrate profile (2013), Tetrahedron, 69, 3561-3564.
No PubMed abstract available

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
-
-
Komagataella pastoris GS115
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.073
-
substrate 1-phenylethanonee, pH 6.5, temperature not specified in the publication Komagataella pastoris
0.074
-
substrate 1-(4-chlorophenyl)ethanone, pH 6.5, temperature not specified in the publication Komagataella pastoris
0.522
-
substrate ethyl 3-oxopentanoate, pH 6.5, temperature not specified in the publication Komagataella pastoris
6.3
-
substrate 4-chlorobenzaldehyde, pH 6.5, temperature not specified in the publication Komagataella pastoris
7
-
substrate 4-fluorobenzaldehyde, pH 6.5, temperature not specified in the publication Komagataella pastoris
10.9
-
substrate 4-bromobenzaldehyde, pH 6.5, temperature not specified in the publication Komagataella pastoris
1502
-
substrate ethyl 4-chloro-3-oxobutanoate, pH 6.5, temperature not specified in the publication Komagataella pastoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(4-chlorophenyl)ethanone + NADPH + H+
-
Komagataella pastoris (S)-1-(4-chlorophenyl)ethanol + NADP+ 86% conversion, 99% enantiomeric excess ?
1-(4-chlorophenyl)ethanone + NADPH + H+
-
Komagataella pastoris GS115 (S)-1-(4-chlorophenyl)ethanol + NADP+ 86% conversion, 99% enantiomeric excess ?
1-phenylethanone + NADPH + H+
-
Komagataella pastoris (S)-1-phenylethanol + NADP+ 60% conversion, 99% enantiomeric excess ?
1-phenylethanone + NADPH + H+
-
Komagataella pastoris GS115 (S)-1-phenylethanol + NADP+ 60% conversion, 99% enantiomeric excess ?
4-bromobenzaldehyde + NADPH + H+ 99% conversion Komagataella pastoris (4-bromophenyl)methanol + NADP+
-
?
4-bromobenzaldehyde + NADPH + H+ 99% conversion Komagataella pastoris GS115 (4-bromophenyl)methanol + NADP+
-
?
4-chlorobenzaldehyde + NADPH + H+ 99% conversion Komagataella pastoris (4-chlorophenyl)methanol + NADP+
-
?
4-chlorobenzaldehyde + NADPH + H+ 99% conversion Komagataella pastoris GS115 (4-chlorophenyl)methanol + NADP+
-
?
4-fluorobenzaldehyde + NADPH + H+ 99% conversion Komagataella pastoris (4-fluorophenyl)methanol + NADP+
-
?
ethyl 3-oxopentanoate + NADPH + H+
-
Komagataella pastoris ethyl (S)-3-hydroxypentanoate + NADP+ 99% conversion, 99% enantiomeric excess ?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ 99% conversion, 92% enantiomeric excess Komagataella pastoris ethyl (R)-4-chloro-3-hydroxybutanoate + NADP+ 99% conversion, 92% enantiomeric excess ?
additional information enzyme is able to reduce both aldehydes and ketones Komagataella pastoris ?
-
?
additional information enzyme is able to reduce both aldehydes and ketones Komagataella pastoris GS115 ?
-
?
phenylglyoxal + 2 NADPH + 2 H+
-
Komagataella pastoris (R)-1-phenyl-1,2-ethanediol + 2 NADP+ 99% yield and 99% enantiomeric excess, by coupling with D-glucose dehydrogenase for the regeneration of cofactor NADPH ?