Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.184 extracted from

  • Li, N.; Ni, Y.; Sun, Z.
    Purification and characterization of carbonyl reductase from Candida krusei SW 2026 involved in enantioselective reduction of ethyl 2-oxo-4-phenylbutyrate (2010), J. Mol. Catal. B, 66, 190-197.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dinitrophenylhydrazine 1mM, 0.9% residual activity Pichia kudriavzevii
2-nitrobenzoate 1mM, 8.6% residual activity Pichia kudriavzevii
Ag+ 1mM, 18% residual activity Pichia kudriavzevii
ascorbic acid 1mM, 14% residual activity Pichia kudriavzevii
citrate 1mM, 48.3% residual activity Pichia kudriavzevii
Cu2+ 1mM, 32% residual activity Pichia kudriavzevii
diethyldicarbonate 1mM, 32.8% residual activity Pichia kudriavzevii
Fe3+ 1mM, 29% residual activity Pichia kudriavzevii
hydroxylamine 1mM, 29.5% residual activity Pichia kudriavzevii
Hydroxyquinoline 1mM, 45% residual activity Pichia kudriavzevii
L-cysteine 1mM, 18.5% residual activity Pichia kudriavzevii
N-ethylmaleimide 1mM, 5.3% residual activity Pichia kudriavzevii
oxalate 1mM, 42.8% residual activity Pichia kudriavzevii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.319
-
ethyl 2-oxo-4-phenylbutanoate pH 7.0, 30°C Pichia kudriavzevii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
gel filtration Pichia kudriavzevii
46000
-
1 * 46000, SDS-PAGE Pichia kudriavzevii

Organism

Organism UniProt Comment Textmining
Pichia kudriavzevii
-
-
-
Pichia kudriavzevii SW 2026
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.6
-
pH 7.0, 30°C Pichia kudriavzevii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-4-phenylbutanoate + NADPH + H+ 77.1% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii (R)-2-hydroxy-4-phenylbutanoate + NADP+
-
?
2-oxo-4-phenylbutanoate + NADPH + H+ 77.1% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii SW 2026 (R)-2-hydroxy-4-phenylbutanoate + NADP+
-
?
ethyl 2-oxo-3-phenylpyruvate + NADPH + H+ 97.7% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii ethyl 2-hydroxy-3-phenylpyruvate + NADP+
-
?
ethyl 2-oxo-3-phenylpyruvate + NADPH + H+ 97.7% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii SW 2026 ethyl 2-hydroxy-3-phenylpyruvate + NADP+
-
?
ethyl 2-oxo-4-phenylbutanoate + NADPH + H+
-
Pichia kudriavzevii ethyl (R)-2-hydroxy-4-phenylbutanoate + NADP+
-
?
ethyl 2-oxo-4-phenylbutanoate + NADPH + H+
-
Pichia kudriavzevii SW 2026 ethyl (R)-2-hydroxy-4-phenylbutanoate + NADP+
-
?
ethyl 4-chloroacetoacetate + NADPH + H+ 76.9% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii ? + NADP+
-
?
ethyl 4-chloroacetoacetate + NADPH + H+ 76.9% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii SW 2026 ? + NADP+
-
?
ethyl benzoylformate + NADPH + H+ 90.8% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii ? + NADP+
-
?
ethyl benzoylformate + NADPH + H+ 90.8% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii SW 2026 ? + NADP+
-
?
methyl 2-oxo-3-phenylpropanoate + NADPH + H+ 86.3% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii methyl 2-hydroxy-3-phenylpropanoate + NADP+
-
?
methyl 2-oxo-4-phenylbutanoate + NADPH + H+ 88.5% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii methyl (R)-2-hydroxy-4-phenylbutanoate + NADP+
-
?
methyl benzoylformate + NADPH + H+ 79.4% of the activity with ethyl 2-oxo-4-phenylbutanoate Pichia kudriavzevii ? + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE Pichia kudriavzevii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Pichia kudriavzevii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35
-
rapid decrease in activity above Pichia kudriavzevii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pichia kudriavzevii

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 more than 80% of maximum activity Pichia kudriavzevii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
highest stability Pichia kudriavzevii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Pichia kudriavzevii