Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C227S | displays a similar kcat, but a 30fold higher Km value for S-nitrosoglutathione, and does not show substrate inhibition | Homo sapiens |
D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P | mutations introduce activity towards S-nitrosoglutathione | Homo sapiens |
P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P | mutations introduce activity towards S-nitrosoglutathione | Homo sapiens |
Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S | mutations introduce activity towards S-nitrosoglutathione | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-nitrosoglutathione | substrate inhibition above 5 * Km, enzyme is covalantly modified. Treatment with dithiothreitol, but not with ascorbic acid, rescues the activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0128 | - |
9,10-phenanthrenequinone | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.028 | - |
S-nitrosoglutathione | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.0354 | - |
9,10-phenanthrenequinone | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.81 | - |
S-nitrosoglutathione | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.832 | - |
S-nitrosoglutathione | mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
1.09 | - |
S-nitrosoglutathione | mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication | Homo sapiens | |
2.93 | - |
S-nitrosoglutathione | mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75828 | isoform CBR 3 | - |
Homo sapiens | P16152 | isoform CBR 1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9,10-phenanthrenequinone + NADPH + H+ | - |
Homo sapiens | ? + NADP+ | - |
? | |
S-nitrosoglutathione + NADPH + H+ | - |
Homo sapiens | ? + NADP+ | - |
? | |
S-nitrosoglutathione + NADPH + H+ | no substrate for wild-type, but substrate for mutants D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, and Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S | Homo sapiens | ? + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBR 1 | - |
Homo sapiens |
CBR 3 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.02 | - |
S-nitrosoglutathione | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
2.62 | - |
S-nitrosoglutathione | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
5.05 | - |
S-nitrosoglutathione | mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication | Homo sapiens | |
5.25 | - |
9,10-phenanthrenequinone | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
10.83 | - |
S-nitrosoglutathione | mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication | Homo sapiens | |
20.45 | - |
S-nitrosoglutathione | mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
36.2 | - |
9,10-phenanthrenequinone | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
S-nitrosoglutathione | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.72 | - |
S-nitrosoglutathione | mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication | Homo sapiens | |
2.5 | - |
S-nitrosoglutathione | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
9.93 | - |
S-nitrosoglutathione | mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication | Homo sapiens | |
24.58 | - |
S-nitrosoglutathione | mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
93.45 | - |
S-nitrosoglutathione | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
148.3 | - |
9,10-phenanthrenequinone | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
2828 | - |
9,10-phenanthrenequinone | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens |