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Literature summary for 1.1.1.184 extracted from

  • Miura, T.; Nishinaka, T.; Takama, M.; Murakami, M.; Terada, T.
    Chinese hamster monomeric carbonyl reductases of the short-chain dehydrogenase/reductase superfamily (2009), Chem. Biol. Interact., 178, 110-116.
    View publication on PubMed
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
pET28a-CHCR-transformed Escherichia coli BL21(DE3)pLys cells Cricetulus griseus

Inhibitors

Inhibitors Comment Organism Structure
NADP+ NADPH-linked reductase activities of CHCRs are partly inhibited by 2 mM NADP+; NADPH-linked reductase activities of CHCRs are partly inhibited by 2 mM NADP+; NADPH-linked reductase activities of CHCRs are partly inhibited by 2 mM NADP+ Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00183
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.00214
-
 NADPH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.0022
-
 NADPH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.0026
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.0218
-
 NADPH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.043
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.078
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.12
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.3
-
 NADH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.338
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.354
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.386
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.43
-
 NADH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.435
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33950
-
 mass spectrometry, His-tagged CHCR2 Cricetulus griseus
34000
-
 mass spectrometry, His-tagged CHCR1 Cricetulus griseus
34040
-
 sequence analysis, His-tagged CHCR1 Cricetulus griseus
34080
-
 sequence analysis, His-tagged CHCR2 Cricetulus griseus
34300
-
 mass spectrometry, His-tagged CHCR3 Cricetulus griseus
34430
-
 sequence analysis, His-tagged CHCR3 Cricetulus griseus
35000
-
 1 * 35000, SDS-PAGE Cricetulus griseus
38000
-
 1 * 38000, SDS-PAGE Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus Q924V2
-
-
Cricetulus griseus Q924V3
-
-
Cricetulus griseus Q9JJN7
-
-

Purification (Commentary)

Purification (Comment) Organism
by metal-chelating affinity column chromatography and gel filtration Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Cricetulus griseus
-
CHO-K1 cell
-
 Cricetulus griseus
-
kidney
-
 Cricetulus griseus
-
kidney high expression of CHCR1 at both the mRNA and protein levels Cricetulus griseus
-
kidney high expression of CHCR2 at both the mRNA and protein levels Cricetulus griseus
-
lung
-
 Cricetulus griseus
-
additional information CHCR1 is absent from uterus and ovary. CHCR1 shows a sex-dependent expression at the protein level Cricetulus griseus
-
ovary
-
 Cricetulus griseus
-
testis high expression of CHCR1 at both the mRNA and protein levels Cricetulus griseus
-
testis high expression of CHCR2 at both the mRNA and protein levels Cricetulus griseus
-
uterus
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyacetophenone + NADH + H+
-
 Cricetulus griseus (S)-1-phenyl-1,2-ethanediol + NAD+
-
 ?
4-benzoylpyridine + NADPH + H+
-
 Cricetulus griseus (S)-phenyl(pyridin-4-yl)methanol + NADP+
-
 ?
4-nitrobenzaldehyde + NADPH
-
 Cricetulus griseus 4-nitrobenzyl alcohol + NADP+
-
 ?
isatin + NADPH
-
 Cricetulus griseus ? + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Cricetulus griseus
monomer 1 * 35000, SDS-PAGE Cricetulus griseus
monomer 1 * 38000, SDS-PAGE Cricetulus griseus

Synonyms

Synonyms Comment Organism
carbonyl reductase
-
 Cricetulus griseus
CHCR1
-
 Cricetulus griseus
CHCR2
-
 Cricetulus griseus
CHCR3
-
 Cricetulus griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0283
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.053
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.3
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.5167
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
0.583
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.03
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.087
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.225
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.967
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Cricetulus griseus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.2
-
 at 2 mM Cricetulus griseus NADP+
2
-
 at 2 mM Cricetulus griseus NADP+