Application | Comment | Organism |
---|---|---|
industry | improved (R)-1-phenyl-1,2-ethanediol production by a codon optimized R-specific carbonyl reductase whose coding gene rcr is engineered by truncating its disorder sequence of 4-27 bp at 5'-terminus and adaption of codon usage bias in Escherichia coli. It will provide a new method to establish an effective expression system for improving chiral alcohol productivity by codon optimization | Candida parapsilosis |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes inserted into vector pQE30a and expressed in Escherichia coli BL21 (AITM) cells | Candida parapsilosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | codon variants DELTA24 (truncation of disorder sequence of 4-27 bp at the 5'-terminus), aRCR (its nine out of 11 rare codons are adapted according to the codon bias in Escherichia coli), and mRCR (its disorder sequence of 4-27 bp at the 5'-terminus is truncated and nine out of 11 rare codons are adapted) are expressed at higher levels than the wild-type. Mutant mRCR presents highest enzyme activity. When compared with wild-type, mRCR shows an increase of 35.6% in the total activity of cell-free extracts | Candida parapsilosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis | A1X808 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes purified to homogeneity by Ni-affinity chromatography | Candida parapsilosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyacetophenone + NADPH + H+ | - |
Candida parapsilosis | (R)-1-phenyl-1,2-ethanediol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
R-specific carbonyl reductase | - |
Candida parapsilosis |