Cloned (Comment) | Organism |
---|---|
overexpression of SSCR in Escherichia coli strain BL21(DE3) | Sporidiobolus salmonicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporidiobolus salmonicolor | - |
red yeast, strain AKU 4429 | - |
Purification (Comment) | Organism |
---|---|
recombinant SSCR from Escherichia coli strain BL21(DE3) | Sporidiobolus salmonicolor |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ | substrate specificity, enantioselectivity, and enzyme-substrate docking studies, Ser133 and Tyr177 are important | Sporidiobolus salmonicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2-dimethylpropiophenone + NADPH + H+ | - |
Sporidiobolus salmonicolor | 2,2-dimethylpropiophenol + NADP+ | over 90% chiral purity of the alcohol product | ? | |
butyrophenone + NADPH + H+ | - |
Sporidiobolus salmonicolor | (1S)-1-phenylbutan-1-ol + NADP+ | over 90% chiral purity of the alcohol product | ? | |
ethyl 3,3-dimethyl-2-oxobutyrate + NADPH + H+ | stereoselective reaction | Sporidiobolus salmonicolor | (S)-ethyl 3,3-dimethyl-2-hydroxybutyrate + NADP+ | over 90% chiral purity of the alcohol product | ? | |
additional information | the enzyme performs enantioselective reductions of sterically bulky aryl alkyl ketones, overview | Sporidiobolus salmonicolor | ? | - |
? | |
phenyl cyclopropyl ketone + NADPH + H+ | - |
Sporidiobolus salmonicolor | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SSCR | - |
Sporidiobolus salmonicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 6.6 | assay at | Sporidiobolus salmonicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Sporidiobolus salmonicolor |