Cloned (Comment) | Organism |
---|---|
sniffer gene, expression as His-tagged enzyme from Escherichia coli strain M15 | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, 0.001 ml water with 0.003 ml protein solution and 0.003 ml reservoir solution giving 18% w/v PEG 4000, 0.15 M sodium acetate, 75 mM Tris, pH 8.5, severeal days at 4°C, X-ray diffraction structure determination and analysis at 1.75 A resolution, molecular modeling | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by nickel affinity chromatography | Drosophila melanogaster |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ | stereospecifc reaction mechanism | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrobenzaldehyde + NADPH | artificial substrate | Drosophila melanogaster | 4-nitrobenzyl alcohol + NADP+ | - |
? | |
9,10-phenanthrenequinone + NADPH | artificial substrate | Drosophila melanogaster | ? + NADP+ | - |
? | |
menadione + NADPH | artificial substrate | Drosophila melanogaster | ? + NADP+ | - |
? | |
additional information | stereospecific enzyme, substrate binding mechanism, active site cleft | Drosophila melanogaster | ? | - |
? | |
pyridin-4-carboxyaldehyde + NADPH | artificial substrate | Drosophila melanogaster | pyridin-4-ylmethanol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase | - |
Drosophila melanogaster |
More | enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | beta-form, cofactor binding site structure | Drosophila melanogaster |