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Literature summary for 1.1.1.183 extracted from
- Biosynthesis of geranial, a potent aroma compound in ginger rhizome (Zingiber officinale): molecular cloning and characterization of geraniol dehydrogenase (2014), Plant Biotechnol., 31, 525-534.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Zingiber officinale |
| gene ZoGeDH, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, reverse transcription real-time RT-PCR enzyme expression analysis | Zingiber officinale |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0609 | - |
geraniol | at pH 9.5 and 25°C | Zingiber officinale |  |
| 0.0609 | - |
geraniol | pH 9.5, 25°C, recombinant enzyme | Zingiber officinale | |
| 0.0926 | - |
nerol | at pH 9.5 and 25°C | Zingiber officinale | |
| 0.0926 | - |
nerol | pH 9.5, 25°C, recombinant enzyme | Zingiber officinale | |
| 0.1424 | - |
NADP+ | at pH 9.5 and 25°C | Zingiber officinale | |
| 0.1424 | - |
NADP+ | pH 9.5, 25°C, recombinant enzyme | Zingiber officinale | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | contains zinc | Zingiber officinale | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39590 | - |
- |
Zingiber officinale |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geraniol + NADP+ | Zingiber officinale | - |
geranial + NADPH + H+ | - |
r | |
| geraniol + NADP+ | Zingiber officinale | 100% activity | geranial + NADPH + H+ | - |
? | |
| additional information | Zingiber officinale | feeding exeriments with cell culture: the incorporated geraniol and geranyl acetate are transformed and detected (by GC-MS) as geranial, geraniol, geranyl acetate and citronellol, while nerol and neral are hardly detected | ? | - |
? | |
| nerol + NADP+ | Zingiber officinale | enzyme GeDH shows equal preference for geraniol and nerol | neral + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zingiber officinale | - |
- |
- |
| Zingiber officinale | A0A0E4B3N6 | cv. Kintoki | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Zingiber officinale | - |
| additional information | expression levels of ZoGeDH in various ginger plant tissues are in accordance with the accumulation of geranial, except in old rhizome | Zingiber officinale | - |
| rhizome | - |
Zingiber officinale | - |
| rhizome | young rhizomes | Zingiber officinale | - |
| stem | - |
Zingiber officinale | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-butanol + NADP+ | 5.4% activity compared to geraniol | Zingiber officinale | butanal + NADPH + H+ | - |
? | |
| 3,7-dimethyloctanol + NADP+ | 11.5% activity compared to geraniol | Zingiber officinale | 3,7-dimethyloctanal + NADPH + H+ | - |
? | |
| 3-methyl-2-buten-1-ol + NADP+ | 22.9% activity compared to geraniol | Zingiber officinale | 3-methyl-2-buten-1-al + NADPH + H+ | - |
? | |
| 3-methyl-2-buten-1-ol + NADP+ | 22.9% activity compared to geraniol | Zingiber officinale | 3-methyl-2-butenal + NADPH + H+ | - |
? | |
| 3-methyl-3-buten-1-ol + NADP+ | 6.9% activity compared to geraniol | Zingiber officinale | 3-methyl-3-buten-1-al + NADPH + H+ | - |
? | |
| 3-methyl-3-buten-1-ol + NADP+ | 6.9% activity compared to geraniol | Zingiber officinale | 3-methyl-3-butenal + NADPH + H+ | - |
? | |
| 3-phenylpropanol + NADP+ | 16.2% activity compared to geraniol | Zingiber officinale | 3-phenylpropanal + NADPH + H+ | - |
? | |
| cinnamyl alcohol + NADP+ | 31.6% activity compared to geraniol | Zingiber officinale | cinnamaldehyde + NADPH + H+ | - |
? | |
| cinnamyl alcohol + NADP+ | 31.6% activity compared to geraniol | Zingiber officinale | cinnamyl aldehyde + NADPH + H+ | - |
? | |
| cis-3-hexenol + NADP+ | 11.9% activity compared to geraniol | Zingiber officinale | cis-3-hexenal + NADPH + H+ | - |
? | |
| citronellol + NADP+ | 16.4% activity compared to geraniol | Zingiber officinale | citronellal + NADPH + H+ | - |
? | |
| geraniol + NADP+ | - |
Zingiber officinale | geranial + NADPH + H+ | - |
r | |
| geraniol + NADP+ | 100% activity | Zingiber officinale | geranial + NADPH + H+ | - |
? | |
| geraniol + NADP+ | enzyme GeDH shows equal preference for geraniol and nerol | Zingiber officinale | geranial + NADPH + H+ | - |
r | |
| hexanol + NADP+ | 24.1% activity compared to geraniol | Zingiber officinale | hexanal + NADPH + H+ | - |
? | |
| additional information | no activity with NAD+ | Zingiber officinale | ? | - |
? | |
| additional information | feeding exeriments with cell culture: the incorporated geraniol and geranyl acetate are transformed and detected (by GC-MS) as geranial, geraniol, geranyl acetate and citronellol, while nerol and neral are hardly detected | Zingiber officinale | ? | - |
? | |
| additional information | the recombinant ZoGeDH catalyzes the NADP+-dependent oxidation from geraniol to citral, a mixture of geranial and neral, but the neral content in vivo is very low. Its substrate specificity is the highest for geraniol and nerol, while that for cinnamyl alcohol is 32% of the activity observed for geraniol | Zingiber officinale | ? | - |
? | |
| n-butanol + NADP+ | 5.4% activity compared to geraniol | Zingiber officinale | butanal + NADPH + H+ | - |
? | |
| nerol + NADP+ | 98.3% activity compared to geraniol | Zingiber officinale | neral + NADPH + H+ | - |
r | |
| nerol + NADP+ | enzyme GeDH shows equal preference for geraniol and nerol | Zingiber officinale | neral + NADPH + H+ | - |
r | |
| phenylethylalcohol + NADP+ | 9.9% activity compared to geraniol | Zingiber officinale | phenylethylaldehyde + NADPH + H+ | - |
? | |
| trans-2-hexenol + NADP+ | 26.6% activity compared to geraniol | Zingiber officinale | trans-2-hexenal + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39590, sequence calculation | Zingiber officinale |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GeDH | - |
Zingiber officinale |
| ZoGeDH | - |
Zingiber officinale |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Zingiber officinale |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.6 | - |
nerol | at pH 9.5 and 25°C | Zingiber officinale | |
| 4.6 | - |
nerol | pH 9.5, 25°C, recombinant enzyme | Zingiber officinale | |
| 5 | - |
NADP+ | at pH 9.5 and 25°C | Zingiber officinale | |
| 5 | - |
NADP+ | pH 9.5, 25°C, recombinant enzyme | Zingiber officinale | |
| 5.4 | - |
geraniol | at pH 9.5 and 25°C | Zingiber officinale | |
| 5.4 | - |
geraniol | pH 9.5, 25°C, recombinant enzyme | Zingiber officinale | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Zingiber officinale |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | 68% of maximal activity is maintained at a pH range of pH 8.0-10.0 | Zingiber officinale |
| 8 | 10 | 68% of the optimum activity is maintained at a pH range of 8.0-10.0 | Zingiber officinale |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity is observed when NAD+ is substituted for NADP+ | Zingiber officinale | |
| NADP+ | - |
Zingiber officinale | |
| NADPH | - |
Zingiber officinale | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Zingiber officinale | sequence calculation | - |
6.79 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenic analysis of ZoGeDH results in its categorization into the cinnamyl alcohol dehydrogenase (CAD) group, along with the previously reported GeDHs of sweet basil (Ocimum basilicum) and wild perilla (P. setoyensis, P. citriodora, and P. frutescens) | Zingiber officinale |
| metabolism | treatment of plants with D2-geraniol accelerates the synthesis of D1-geraniol and D1-geranial. D1-geranial comprises 33.7% of the endogenous geranial content. Other than geraniol, citronellol is also detected as a mixture of D2- and D1-labeled compounds. On the other hand, nerol and neral, cis-isomers of geraniol and nerol, respectively, are hardly detected in either the control or treated plants. These results indicate that geraniol and geranyl acetate are possible precursors for the formation of geranial and citronellol. Proposed biotransformation of geraniol-related compounds in ginger rhizomes, overview | Zingiber officinale |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35 | - |
NADP+ | at pH 9.5 and 25°C | Zingiber officinale | |
| 35 | - |
NADP+ | pH 9.5, 25°C, recombinant enzyme | Zingiber officinale | |
| 50 | - |
nerol | at pH 9.5 and 25°C | Zingiber officinale | |
| 50 | - |
nerol | pH 9.5, 25°C, recombinant enzyme | Zingiber officinale | |
| 89 | - |
geraniol | at pH 9.5 and 25°C | Zingiber officinale | |
| 89 | - |
geraniol | pH 9.5, 25°C, recombinant enzyme | Zingiber officinale | |
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